Tg(H2-Ea-Ins2)1Wehi Transgene Detail MGI Mouse (MGI:3590216)

Tg(H2-Ea-Ins2)1Wehi
Transgene Detail

Summary

Symbol:	Tg(H2-Ea-Ins2)1Wehi
Name:	transgene insertion 1, William and Eliza Hall Institute
MGI ID:	MGI:3590216
Transgene:	Tg(H2-Ea-Ins2)1Wehi Location: unknown
Alliance:	Tg(H2-Ea-Ins2)1Wehi page

Transgene
origin

Strain of Origin:

NOD/ShiLt

Transgene
description

Transgene Type:

Transgenic (Inserted expressed sequence)

Mutation:

Insertion

Tg(H2-Ea-Ins2)1Wehi expresses 1 gene

Mutation details: The transgene comprises nucleotides -1,903 through -39 of the 5' flanking sequence of the MHC Class II gene H2-Ea (k allele), containing the promoter, followed by a 909 base pair mouse (NOD strain) genomic DNA fragment containing the sequence encoding prepronsulin II and the SV40 small t intron and polyadenylation signal.

Transgene-derived mRNA is detectable in spleen and thymus of transgenic NOD/LtWehi mice, but not of control littermates, by reverse transcription followed by PCR (RT/PCR) using either primers for H2-Ea^k and proinsulin or two proinsulin primers. Immunoreactive proinsulin is detectable in medium from cultured transgenic splenocytes only after 8 days' culture and at levels 10^-2 - 10^-3 times those of cultured islets or islet cells; levels are increased by culture with interferon gamma and tumor necrosis factor alpha. (J:100232)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two lines of mice were created with this trasgenic construct on the NOD background. No distinction was made between them.

References

Original:	J:100232 French MB, et al., Transgenic expression of mouse proinsulin II prevents diabetes in nonobese diabetic mice. Diabetes. 1997 Jan;46(1):34-9
All:	8 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23