Antibody:	anti-alpha1C subunit
Detection system:	Secondary antibody coupled to alkaline phosphatase
Assay notes:	The authors used either this antibody or an anti-II-III loop antibody (MGI:3590161) and stated that similar results were obtained with both.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Weak	Uniform

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Uniform	1a

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present	Uniform	1b
TS19: atrioventricular canal	Absent		1b
TS19: endocardial cushion	Absent		1b
TS19: outflow tract	Absent		1b
TS19: outflow tract aortic component	Absent		1b
TS19: outflow tract pulmonary component	Absent		1b

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present	Uniform	1c
TS20: atrioventricular endocardial cushion	Absent		1c
TS20: endocardial cushion	Absent		1c
TS20: outflow tract aortic component	Absent		1c
TS20: outflow tract pulmonary component	Absent		1c

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Present	Uniform	1d
TS24: heart	Present	Uniform	1d	This includes the ventricular conduction system, the atrioventricular node. the bundle of His and the bundle branches.

Structure	Level	Pattern	Image	Note
TS26: atrium cardiac muscle	Present	Uniform	1e
TS26: heart	Present	Uniform	1e	his includes the ventricular conduction system, the atrioventricular node. the bundle of His and the bundle branches.

Structure	Level	Pattern	Image	Note
TS28: heart	Present	Uniform	2a	Homogeneous expression in the myocardium.
TS28: aorta	Absent		2a	Absent in smooth muscle cells.
TS28: pulmonary artery	Absent		2a	Absent in smooth muscle cells.