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Gene Expression Data
Assay Details
Assay
Reference: J:96550 Williams TM, et al., Candidate downstream regulated genes of HOX group 13 transcription factors with and without monomeric DNA binding capability. Dev Biol. 2005 Mar 15;279(2):462-80
Assay type: RNA in situ
MGI Accession ID: MGI:3588178
Gene symbol: Igfbp4
Gene name: insulin-like growth factor binding protein 4
Probe: Igfbp4 cDNA5
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4L: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: forelimb interdigital region Present 4L

Specimen 5A: embryonic day 12.5 (more )
Note: Stage 7 limb bud.
Structure Level Pattern Image Note
TS20: hand Strong Regionally restricted 5A Strong expression in proximal forelimb bud with weaker expression throughout the distal autopod.

Specimen 5B: embryonic day 12.5 (more )
Note: Stage 8 limb bud.
Structure Level Pattern Image Note
TS20: hand Present Regionally restricted 5B Expression detected in proximal limb bud.
TS20: forelimb interdigital region Present 5B

Specimen 5C: embryonic day 12.5 (more )
Note: Stage 9 limb bud.
Structure Level Pattern Image Note
TS20: interdigital region between forelimb digits 1 and 2 Strong 5C
TS20: interdigital region between forelimb digits 4 and 5 Strong 5C
TS20: interdigital region between forelimb digits 2 and 3 Weak 5C
TS20: interdigital region between forelimb digits 3 and 4 Weak 5C

Specimen 5D: embryonic day 12.5; Hoxa13tm2Ipc/Hoxa13tm2Ipc (more )
Note: Stage 7 limb bud.
Structure Level Pattern Image Note
TS20: hand Strong Regionally restricted 5D Strong expression in proximal forelimb bud with weaker expression throughout the distal autopod as in wild type.

Specimen 5E: embryonic day 12.5; Hoxa13tm2Ipc/Hoxa13tm2Ipc (more )
Note: Stage 8 limb bud.
Structure Level Pattern Image Note
TS20: hand Present Regionally restricted 5E Expression is detected in the proximal forelimb bud with weaker expression in the interdigital regions.
TS20: forelimb interdigital region Weak Regionally restricted 5E Expression in the mutant interdigital regions appears much weaker compared to wild type.

Specimen 5F: embryonic day 12.5; Hoxa13tm2Ipc/Hoxa13tm2Ipc (more )
Note: Stage 9 limb bud.
Structure Level Pattern Image Note
TS20: interdigital region between forelimb digits 1 and 2 Absent 5F
TS20: interdigital region between forelimb digits 2 and 3 Present 5F
TS20: interdigital region between forelimb digits 3 and 4 Present 5F
TS20: interdigital region between forelimb digits 4 and 5 Weak 5F


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory