Probe:	Fstl cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Present		4I

Structure	Level	Pattern	Image	Note
TS20: interdigital region between hindlimb digits 2 and 3	Present		6A	The expression level in interdigital region 2 and 3 was greater than in interdigital region 4.
TS20: interdigital region between hindlimb digits 3 and 4	Present		6A	The expression level in interdigital region 2 and 3 was greater than in interdigital region 4.
TS20: interdigital region between hindlimb digits 4 and 5	Present		6A	The expression level in interdigital region 2 and 3 was greater than in interdigital region 4.

Structure	Level	Pattern	Image	Note
TS21: interdigital region between forelimb digits 1 and 2	Weak		6B	Expression was high in the posterior interdigital region 4 relative to interdigital regions 1-3.
TS21: interdigital region between forelimb digits 2 and 3	Weak		6B	Expression was high in the posterior interdigital region 4 relative to interdigital regions 1-3.
TS21: interdigital region between forelimb digits 3 and 4	Weak		6B	Expression was high in the posterior interdigital region 4 relative to interdigital regions 1-3.
TS21: interdigital region between forelimb digits 4 and 5	Strong	Regionally restricted	6B	Expression was high in the posterior interdigital region 4 relative to interdigital regions 1-3.

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region	Weak	Regionally restricted	6C	Expression was attenuated compared to wild type littermates. The degree of loss was variable, however interdigital region 4 expression was always reduced.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region	Weak	Regionally restricted	6D	Expression was attenuated compared to wild type littermates. The degree of loss was variable, however interdigital region 4 expression was always reduced.