Antibody:	DENTT antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	7A	Expression is in the trophoblasts of the ectoplacental cone.
TS28: decidua	Strong	Regionally restricted	7A	Expression is in the trophoblasts of the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	7B	Expression is in the trophoblasts of the ectoplacental cone.
TS28: decidua	Strong	Regionally restricted	7B	Expression is in the trophoblasts of the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS15: extraembryonic component	Very strong	Regionally restricted	7C	Expression is in the nuclei of the giant trophoblasts.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted	7E	Expression is in the outer layer of the amniotic membrane.

Structure	Level	Pattern	Image	Note
TS17: extraembryonic component	Weak	Regionally restricted		Absent in most giant trophoblasts.

Structure	Level	Pattern	Image	Note
TS19: heart atrium	Present		7F	Expression is nuclear.
TS19: heart ventricle	Present		7F	Expression is nuclear.

Structure	Level	Pattern	Image	Note
TS17: heart	Present		7G	Expression is nuclear.

Structure	Level	Pattern	Image	Note
TS22: heart	Absent		7H

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	7I	Expression is in the endothelium. Shown is the brain.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		7J

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		7K

Structure	Level	Pattern	Image	Note
TS24: forebrain	Present	Regionally restricted	7L	Expression is in the hippocampus.

Structure	Level	Pattern	Image	Note
TS24: ventral grey horn	Present		7M

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present	Uniform	7N

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present	Regionally restricted	7O	Only a subset of the neuronal population stained positive.

Structure	Level	Pattern	Image	Note
TS19: future neurohypophysis	Present	Regionally restricted	7P	Expression is in the anterior pituitary.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present		7Q

Structure	Level	Pattern	Image	Note
TS13: heart	Weak
TS13: future brain	Weak

Structure	Level	Pattern	Image	Note
TS15: heart	Weak
TS15: future brain	Present

Structure	Level	Pattern	Image	Note
TS17: heart	Present
TS17: brain	Present
TS17: gut	Weak	Regionally restricted		Expression is in the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS19: heart	Present
TS19: blood vessel	Present	Regionally restricted		Expression is in the endothelium.
TS19: brain	Weak			Expression was stronger in the choroid plexus.
TS19: future spinal cord	Present
TS19: dorsal root ganglion	Weak
TS19: future neurohypophysis	Weak
TS19: pre-cartilage condensation	Weak	Regionally restricted		Expression is in pre-cartilaginous blastema.
TS19: surface ectoderm	Weak	Regionally restricted		Expression is in the epidermis.
TS19: tongue	Weak	Regionally restricted		Expression is in the epithelium.
TS19: intestine	Present	Regionally restricted		Expression is in the epithelium.
TS19: liver	Weak	Regionally restricted		Expression is in hepatocytes.
TS19: metanephros	Present	Regionally restricted		Expression is in the metanephric duct derivatives, absent in the tubules and glomeruli.
TS19: endocrine gland	Weak	Regionally restricted		Weak expression in the secretory cells of the adrenals.

Structure	Level	Pattern	Image	Note
TS20: heart	Weak
TS20: blood vessel	Present	Regionally restricted		Expression is in the endothelium.
TS20: brain	Present
TS20: choroid plexus	Strong
TS20: spinal cord	Present
TS20: dorsal root ganglion	Present
TS20: skeletal muscle	Present
TS20: olfactory epithelium	Weak
TS20: pre-cartilage condensation	Weak	Regionally restricted		Expression is in pre-cartilaginous blastema.
TS20: cartilage	Weak
TS20: epidermis	Weak
TS20: pancreas	Weak
TS20: intestine	Present	Regionally restricted		Expression is in the epithelium.
TS20: tongue	Present	Regionally restricted		Expression is in the epithelium.
TS20: liver	Present	Regionally restricted		Expression is in hepatocytes and is weak in megakaryocytes.
TS20: lung	Weak	Regionally restricted		Weak expression in the airways, absent in alveolar region.
TS20: metanephros	Absent			Expression is absent in the tubules and glomeruli.
TS20: endocrine gland	Weak	Regionally restricted		Weak expression in the secretory cells of the adrenals.

Structure	Level	Pattern	Image	Note
TS21: heart	Absent
TS21: blood vessel	Present	Regionally restricted		Expression is in the endothelium.
TS21: brain	Weak
TS21: choroid plexus	Present
TS21: dorsal root ganglion	Present
TS21: pituitary gland	Weak
TS21: olfactory epithelium	Weak
TS21: cartilage	Weak
TS21: pancreas	Weak
TS21: epidermis	Present
TS21: skeletal muscle	Present
TS21: tongue epithelium	Present
TS21: liver	Present	Regionally restricted		Expression is in hepatocytes and in megakaryocytes.
TS21: lung	Weak	Regionally restricted		Weak expression in the airways, absent in alveolar region.
TS21: metanephros	Weak	Regionally restricted		Expression is in the metanephric duct derivatives and in the tubules and is absent in glomeruli.
TS21: adrenal gland	Present	Regionally restricted		Expression is in the secretory cells of the adrenal gland.

Structure	Level	Pattern	Image	Note
TS22: heart	Absent
TS22: blood vessel	Present	Regionally restricted		Expression is in the endothelium.
TS22: brain	Weak
TS22: choroid plexus	Present
TS22: spinal cord	Weak
TS22: dorsal root ganglion	Present
TS22: pancreas	Present
TS22: skeletal muscle	Present
TS22: olfactory epithelium	Weak
TS22: cartilage	Strong
TS22: tongue epithelium	Strong
TS22: epidermis	Weak
TS22: tooth	Weak
TS22: vibrissa	Weak
TS22: intestine	Present	Regionally restricted		Expression is in the epithelium.
TS22: liver	Present	Regionally restricted		Expression is weak in hepatocytes and stronger in megakaryocytes.
TS22: lung	Weak	Regionally restricted		Weak expression in the airways, absent in alveolar region.
TS22: early nephron	Strong
TS22: maturing glomerular tuft	Absent
TS22: adrenal gland	Strong	Regionally restricted		Expression is in the secretory cells.
TS22: testis	Weak	Regionally restricted		Expression is in the tubules.

Structure	Level	Pattern	Image	Note
TS23: heart	Absent
TS23: blood vessel	Weak			Expression is in the endothelium.
TS23: brain	Weak
TS23: choroid plexus	Present
TS23: spinal cord	Present
TS23: dorsal root ganglion	Weak
TS23: pituitary gland	Weak
TS23: olfactory epithelium	Present
TS23: pancreas	Present
TS23: salivary gland	Present
TS23: skeletal muscle	Present
TS23: tongue epithelium	Present
TS23: vibrissa	Present
TS23: cochlear duct epithelium	Weak
TS23: cartilage	Strong
TS23: seminiferous cord	Strong
TS23: epidermis	Weak
TS23: tooth	Weak
TS23: intestine	Strong	Regionally restricted		Expression is in the epithelium.
TS23: liver	Present	Regionally restricted		Expression is in hepatocytes and stronger in megakaryocytes.
TS23: lung	Present	Regionally restricted		Expression is in the airways, absent in alveolar region.
TS23: early nephron	Strong
TS23: maturing glomerular tuft	Absent
TS23: adrenal gland	Present	Regionally restricted		Expression is in the secretory cells.

Structure	Level	Pattern	Image	Note
TS24: heart	Absent
TS24: blood vessel	Present	Regionally restricted		Expression is in the endothelium.
TS24: brain	Present
TS24: choroid plexus	Present
TS24: spinal cord	Present
TS24: dorsal root ganglion	Weak
TS24: pituitary gland	Present
TS24: olfactory epithelium	Present
TS24: cartilage	Present			Expression is stronger in hypertrophic cartilage.
TS24: skeleton	Strong
TS24: tongue epithelium	Strong
TS24: vibrissa	Strong
TS24: epidermis	Present
TS24: pancreas	Present
TS24: salivary gland	Present
TS24: skeletal muscle	Present
TS24: thymus	Present
TS24: tooth	Present
TS24: intestine	Strong	Regionally restricted		Expression is in the epithelium.
TS24: liver	Present	Regionally restricted		Expression is in hepatocytes and in megakaryocytes.
TS24: lung	Present	Regionally restricted		Expression is in the airways.
TS24: left lung alveolar system	Absent

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong		8A

Structure	Level	Pattern	Image	Note
TS24: skeleton	Very strong	Regionally restricted	8B	Epression is in growth plates.

Structure	Level	Pattern	Image	Note
TS24: skeleton	Very strong	Regionally restricted	8C	Expression is in osteoblasts.

Structure	Level	Pattern	Image	Note
TS24: dental lamina	Strong		8D
TS24: enamel organ	Strong		8D

Structure	Level	Pattern	Image	Note
TS24: tongue epithelium	Very strong		8E

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Very strong		8F

Structure	Level	Pattern	Image	Note
TS23: intestine	Strong	Regionally restricted	8G	Expression is in the pseudostratified epithelium and less intense staining in the muscle layer.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Moderate	Regionally restricted	8H	Expression is in the islets and ducts.

Structure	Level	Pattern	Image	Note
TS23: liver	Very strong	Regionally restricted	8I	Expression is in the megakaryocytes.

Structure	Level	Pattern	Image	Note
TS20: lung	Present	Regionally restricted	8J	Expression is in the airways.
TS20: lung mesenchyme	Absent		8J

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present		8K

Structure	Level	Pattern	Image	Note
TS19: metanephros	Present	Regionally restricted	8L	Expression is in the metanephric duct.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Weak		8M
TS21: adrenal gland	Present		8M

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present		8N
TS22: adrenal gland	Weak		8N

Structure	Level	Pattern	Image	Note
TS22: testis	Weak	Regionally restricted	8O	Expression is in the periphery of the seminiferous tubules.

Structure	Level	Pattern	Image	Note
TS23: seminiferous cord	Very strong		8P	Nuclear staining.
TS23: rete testis	Present		8P	Cytoplasmic staining.
TS23: male reproductive system	Present		8P	Cytoplasmic staining in the epididymus.