Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:99396
Vitale A, et al., Mouse cPLA2gamma, a novel oocyte and early embryo-abundant phospholipase A2 gamma-like protein, is targeted to the nuclear envelope during germinal vesicle breakdown. Dev Biol. 2005 Jun 15;282(2):374-84
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3587184
|
|
Gene symbol:
|
Pla2g4c
|
|
Gene name:
|
phospholipase A2, group IVC (cytosolic, calcium-independent)
|
|
Antibody:
|
Anti-PLA2g
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
8B:
postnatal adult
(more  )
Note: Specimen is Germinal Vesicle.
Specimen
8B:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Specimen is Germinal Vesicle.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
8B
|
Expression is detected in the cortical region and nucleoplasm.
|
Specimen
8C:
postnatal adult
(more )
Note: Specimen is Pronuclear oocyte.
Specimen
8C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Female
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Specimen is Pronuclear oocyte.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
8C
|
Expression is detected in the cortical region and nucleoplasm.
|
Specimen
8D:
embryonic day 1.0
(more )
Specimen
8D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 1.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
8D
|
Expression is detected in the cortical region and nucleoplasm. In 2-cell and 4-8-cell embryos blastomere cortical staining is mainly limited to regions that are in contact with the extrecellular space.
|
Specimen
8E:
embryonic day 2.5
(more )
Specimen
8E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 2.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-8 cell stage embryo |
Present |
Regionally restricted |
8E
|
Expression is detected in the cortical region and nucleoplasm. In 2-cell and 4-8-cell embryos blastomere cortical staining is mainly limited to regions that are in contact with the extrecellular space.
|
Specimen
8F:
embryonic day 3.0
(more )
Specimen
8F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 3.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Weak |
Regionally restricted |
8F
|
Expression is detected in the cortical region and nucleoplasm.
|
Specimen
8G:
embryonic day 4.0
(more )
Specimen
8G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 4.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: inner cell mass |
Weak |
Regionally restricted |
8G
|
Expression is detected in the cortical region and nucleoplasm.
|
Specimen
6:
postnatal adult
(more )
Specimen
6:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
|
6
|
|
| TS28: ovary follicle |
Ambiguous |
|
6
|
Weak staining of stromal tissue surrounding follicle cells was observed but authors state it probably was non-specific.
|
|
Analysis Tools