Antibody:	anti-mouse VEGF
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: lung epithelium	Present		4B , 4C
TS19: lung mesenchyme	Present	Regionally restricted	4C , 4B	Expression is in the subepithelial matrix of all airways.
TS19: blood vessel	Present	Regionally restricted	4C , 4B	Expression is in the lumenal surface of blood vessels. Shown are the vessels of the lung.

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present	Regionally restricted	4F , 4E	Staining is absent in the proximal subepithelial matrix, but is present in the surrounding distal subepithelial matrix.
TS21: lung epithelium	Present	Graded	4F , 4E	Increased staining in the distal airways.
TS21: blood vessel	Present	Regionally restricted	4E , 4F	Expression is in the lumenal surface of blood vessels. Shown are the vessels of the lung.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		4H , 4I
TS23: lung mesenchyme	Strong	Regionally restricted	4H , 4I	Expression is in the subepithelial matrix, absent in the proximal airways.
TS23: blood vessel	Present	Regionally restricted	4H , 4I	Expression is in the lumenal surface of blood vessels. Shown are the vessels of the lung.