Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(TcraCl4,TcrbCl4)1Shrm
Name:	transgene insertion 1, Linda Sherman
MGI ID:	MGI:3586582
Synonyms:	C4, CL4, CL-4, CL-4 TCR, CL4-TCR, Clone 4, clone 4 TCR, Clone-4 TCR, (HA)-TCR
Transgene:	Tg(TcraCl4,TcrbCl4)1Shrm  Location: unknown
Alliance:	Tg(TcraCl4,TcrbCl4)1Shrm page

Strain of Origin:

(C57BL/6 x BALB/c)F1

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(TcraCl4,TcrbCl4)1Shrm expresses 2 genes Transgene expresses: Organism Expressed Gene Note mouse Tcra (MGI:98553) mouse Tcrb (MGI:98578)   Mutation details: Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc1 H2d H2-t18c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer EH, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, EH, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2kd. (J:90982, J:99756)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available

Original:	J:99756 Morgan DJ, et al., CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol. 1996 Aug 1;157(3):978-83
All:	84 reference(s)