Gene Expression Data
Assay Details
Assay
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Reference:
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J:93300
Blackshaw S, et al., Genomic analysis of mouse retinal development. PLoS Biol. 2004 Oct;2(9):E247
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3585877
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Gene symbol:
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Gnao1
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Gene name:
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guanine nucleotide binding protein, alpha O
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Probe:
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IMAGE clone 480073
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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This data is from Supplemental Table 5, Gene column, row: 30209 guanine nucleotide binding protein, alpha o . After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.
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Results
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Specimen
E14:
embryonic day 14.0
(more )
Specimen
E14:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Weak |
Regionally restricted |
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Weak expression was tentatively detected in a subset of cells in the outer neuroblastic layer.
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Specimen
E16:
embryonic day 16.0
(more )
Specimen
E16:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Weak |
Regionally restricted |
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Weak expression was tentatively detected in a subset of cells in the outer neuroblastic layer.
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Specimen
E18:
embryonic day 18.0
(more )
Specimen
E18:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: retina |
Weak |
Regionally restricted |
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Weak expression was tentatively detected in a subset of cells in the outer neuroblastic layer.
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Specimen
P0/1:
postnatal day 0-1
(more )
Specimen
P0/1:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 0-1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: retina inner nuclear layer |
Present |
Regionally restricted |
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Expression was detected in a subset of cells in the scleral inner neuroblastic layer.
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Specimen
P2/3:
postnatal day 2-3
(more )
Specimen
P2/3:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
P4/5:
postnatal day 4-5
(more )
Specimen
P4/5:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 4-5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
Regionally restricted |
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Expression was detected in the vitreal and scleral inner neuroblastic layer. The expression level was higher in the vitreal inner neuroblastic layer.
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Specimen
P6/7:
postnatal day 6-7
(more )
Specimen
P6/7:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 6-7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
P8/10:
postnatal day 8-10
(more )
Specimen
P8/10:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 8-10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
Adult:
postnatal adult
(more )
Specimen
Adult:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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