Gene Expression Data
Assay Details
Assay
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Reference:
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J:93300
Blackshaw S, et al., Genomic analysis of mouse retinal development. PLoS Biol. 2004 Oct;2(9):E247
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3585847
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Gene symbol:
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Cdkn1b
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Gene name:
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cyclin dependent kinase inhibitor 1B
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Probe:
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UI-M-AN1-afc-d-07-0-UI.s1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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This data is from Supplemental Table 5, Gene column, row: 2958 Cdkn1b cyclin-dependent kinase inhibitor 1B (P27). After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.
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Results
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Specimen
E16:
embryonic day 16.0
(more )
Specimen
E16:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: retina nuclear layer |
Present |
Regionally restricted |
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Expression was detected in the outer neuroblastic layer.
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Specimen
E18:
embryonic day 18.0
(more )
Specimen
E18:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
P0/1:
postnatal day 0-1
(more )
Specimen
P0/1:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 0-1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: retina outer nuclear layer |
Present |
Regionally restricted |
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Expression was detected in the vitreal outer neuroblastic layer.
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Specimen
P2/3:
postnatal day 2-3
(more )
Specimen
P2/3:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 2-3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: retina outer nuclear layer |
Present |
Regionally restricted |
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Expression was detected in the vitreal outer neuroblastic layer.
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Specimen
P4/5:
postnatal day 4-5
(more )
Specimen
P4/5:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 4-5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina outer nuclear layer |
Present |
Regionally restricted |
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Expression was detected in the vitreal outer neuroblastic layer.
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Specimen
P6/7:
postnatal day 6-7
(more )
Specimen
P6/7:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 6-7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
P8/10:
postnatal day 8-10
(more )
Specimen
P8/10:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 8-10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
Adult:
postnatal adult
(more )
Specimen
Adult:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Present |
Regionally restricted |
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Expression was detected in the Muller glia.
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Specimen
CNS:
postnatal day 6
(more )
Specimen
CNS:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
Embryo:
embryonic day 16.0
(more )
Specimen
Embryo:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: forebrain |
Present |
Regionally restricted |
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Expression was detected in hippocampus.
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TS24: cerebellum |
Present |
Regionally restricted |
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Expression was detected in the external granule layer of the developing cerebellum.
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