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Gene Expression Data
Assay Details
Assay
Reference: J:93300 Blackshaw S, et al., Genomic analysis of mouse retinal development. PLoS Biol. 2004 Oct;2(9):E247
Assay type: RNA in situ
MGI Accession ID: MGI:3585744
Gene symbol: Spc25
Gene name: SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Probe: IMAGE clone 585541
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: This data is from Supplemental Table 5, Gene column, row: 26062 AD024 protein (endosomal/kinesin-like?). After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.
Results
Specimen E14: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: neural retinal epithelium Present Regionally restricted 5B ADO24 E14 , Submitted Image - U16e14 Expression was detected in the outer neuroblastic layer but was higher in a subset of cells.

Specimen E16: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: retina nuclear layer Present Regionally restricted Submitted Image - U16e16 , 5B ADO24 E16 Expression was detected in the outer neuroblastic layer but was higher in a subset of cells.

Specimen E18: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: retina outer nuclear layer Present Regionally restricted Submitted Image - U16e18 , 5B ADO24 E18 Expression was detected in the outer neuroblastic layer but was higher in a subset of cells.

Specimen P0/1: postnatal day 0-1 (more )
Structure Level Pattern Image Note
TS27: retina outer nuclear layer Present Regionally restricted Submitted Image - U16p0 , 5B ADO24 P0 Expression was detected in scleral and vitreal outer neuroblastic layer. The expression level was higher in the vitreal outer neuroblastic layer.

Specimen P2/3: postnatal day 2-3 (more )
Structure Level Pattern Image Note
TS27: retina outer nuclear layer Present Regionally restricted 5B ADO24 P2 , Submitted Image - U16p2 Expression was detected in the vitreal and scleral outer neuroblastic layer. The expression level was higher in the vitreal outer neuroblastic layer.

Specimen P4/5: postnatal day 4-5 (more )
Structure Level Pattern Image Note
TS28: retina outer nuclear layer Present Regionally restricted 5B ADO24 P4 , Submitted Image - U16p4 Expression was detected in the scleral and vitreal outer neuroblastic layer. The expression level was higher in the vitreal outer neuroblastic layer.

Specimen P6/7: postnatal day 6-7 (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted Submitted Image - U16p6 , 5B ADO24 P6 Expression was detected in the Muller glia.
TS28: retina outer nuclear layer Weak 5B ADO24 P6 , Submitted Image - U16p6

Specimen P8/10: postnatal day 8-10 (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted Submitted Image - U16p8 Expression was detected in the Muller glia.
TS28: retina outer nuclear layer Weak Submitted Image - U16p8

Specimen Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 5B ADO24 Adult , Submitted Image - U16ad , Submitted Image - U16adb Expression was detected in the Muller glia.

Specimen CNS: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: central nervous system Absent

Specimen Embryo: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: diencephalon Present Regionally restricted Expression was detected in diencephalon progenitors.
TS24: embryo Present Expression was widespread.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory