Gba2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93300 Blackshaw S, et al., Genomic analysis of mouse retinal development. PLoS Biol. 2004 Oct;2(9):E247
Assay type:	RNA in situ
MGI Accession ID:	MGI:3585643
Gene symbol:	Gba2
Gene name:	glucosidase beta 2

Probe:	UI-M-AP0-abn-g-07-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	This data is from Supplemental Table 5, Gene column, row: 229444 Gba2 glucosidase beta 2. After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.

Results

Specimen E14: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: retina	Absent

Specimen E16: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: retina	Absent

Specimen E18: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		Submitted Image - 11aE18 , Submitted Image - 11aE18B	Expression was detected in the inner neuroblastic layer.

Specimen P0/1: postnatal day 0-1 (more

)

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present		Submitted Image - 11aP0.5 , Submitted Image - 11aP1.5	Expression was detected in the inner neuroblastic layer.

Specimen P2/3: postnatal day 2-3 (more

)

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present		Submitted Image - 11aP2.5 , Submitted Image - 11aP2.5B	Expression was detected in the inner neuroblastic layer.
TS27: retina outer nuclear layer	Present	Regionally restricted	Submitted Image - 11aP2.5B , Submitted Image - 11aP2.5	Expression was detected in a subset of cells in the vitreal outer neuroblastic layer.

Specimen P4/5: postnatal day 4-5 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		Submitted Image - 11aP5.5 , Submitted Image - 11aP4.5	Expression was detected in the inner neuroblastic layer.
TS28: retina outer nuclear layer	Present	Regionally restricted	Submitted Image - 11aP4.5 , Submitted Image - 11aP5.5	Expression was detected in a subset of cells in the vitreal outer neuroblastic layer.

Specimen P6/7: postnatal day 6-7 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	Submitted Image - 11aP6.5	Expression was detected in amacrine cells.
TS28: retina ganglion cell layer	Present		Submitted Image - 11aP6.5
TS28: retina inner nuclear layer	Present	Regionally restricted	Submitted Image - 11aP6.5	Expression was detected in a subset of cells in the inner nuclear layer.

Specimen P8/10: postnatal day 8-10 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		Submitted Image - 11aP10.5 , Submitted Image - 11aP8.5 , Submitted Image - 11aP9.5
TS28: retina layer	Present	Regionally restricted	Submitted Image - 11aP10.5 , Submitted Image - 11aP8.5 , Submitted Image - 11aP9.5	Expression was detected in horizontal and amacrine cells.
TS28: retina ganglion cell layer	Present		Submitted Image - 11aP10.5 , Submitted Image - 11aP9.5 , Submitted Image - 11aP8.5

Specimen Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	Submitted Image - 11aAdult	Expression was detected in horizontal and amacrine cells.
TS28: retina inner nuclear layer	Present		Submitted Image - 11aAdult

Specimen CNS: postnatal day 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: central nervous system	Strong			Expression was widespread.

Specimen Embryo: embryonic day 16.0 (more

)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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