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Gene Expression Data
Assay Details
Assay
Reference: J:93300 Blackshaw S, et al., Genomic analysis of mouse retinal development. PLoS Biol. 2004 Oct;2(9):E247
Assay type: RNA in situ
MGI Accession ID: MGI:3585619
Gene symbol: Polr2f
Gene name: polymerase (RNA) II (DNA directed) polypeptide F
Probe: UI-M-AI0-aan-d-07-0-UI.s1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: This data is from Supplemental Table 5, Gene column, row: 274860 ESTs, Highly similar to DNA-DIRECTED RNA POLYMERASE II 14.4 KD POLYPEPTIDE. After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.
Results
Specimen E12: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: retina Weak Uniform Expression was panretinal.

Specimen E14: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: neural retinal epithelium Present Regionally restricted Submitted Image - AH1e14.5100 Expression was detected in the inner neuroblastic layer but was weak in the outer neuroblastic layer.

Specimen E16: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: retina nuclear layer Weak Regionally restricted Expression was detected in the outer and inner neuroblastic layers. The expression level was higher in the inner layer.

Specimen E18: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: retina outer nuclear layer Present Submitted Image - AH1e18.5 Expression was detected in the outer neuroblastic layer.
TS26: retina inner nuclear layer Present Submitted Image - AH1e18.5 Expression was detected in the inner neuroblastic layer.

Specimen P0/1: postnatal day 0-1 (more )
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present Regionally restricted Submitted Image - AH1p1.5 Expression was detected in the vitreal inner neuroblastic layer.

Specimen P2/3: postnatal day 2-3 (more )
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present Regionally restricted Submitted Image - AH1p3.5 Expression was detected in the vitreal inner neuroblastic layer.

Specimen P4/5: postnatal day 4-5 (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted Expression was panretinal, but the level was higher in the vitreal inner neuroblastic layer.

Specimen P6/7: postnatal day 6-7 (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted Submitted Image - AH1p6.5 Expression was panretinal, but the level was higher in the ganglion layer.

Specimen P8/10: postnatal day 8-10 (more )
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted Expression was panretinal, but the level was higher in amacrine and ganglion cells.

Specimen CNS: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: central nervous system Present Expression was widespread.

Specimen Embryo: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: embryo Present Expression was widespread.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory