Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Ren1-GFP)2Kwg
Name:	transgene insertion 2, Kenneth W Gross
MGI ID:	MGI:3583367
Synonyms:	Tg(Ren-GFP)2Kwg, Tg(RP23-88k7/Ren-GFP)1Kwg
Transgene:	Tg(Ren1-GFP)2Kwg  Location: unknown  Genetic Position: Chr3, Syntenic
Alliance:	Tg(Ren1-GFP)2Kwg page

Strain of Origin:

(C57BL/10-Oca2p-d/Ros x C3H/HeRos)F1

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The transgene was created by targeted insertion of a "humanized" green fluorescent protein (GFP) coding sequence into the bacterial artificial chromosome (BAC) RP23-88K7. The GFP sequence integrated at position +15 of Ren1, which is centered within the approximately 250-kb BAC, with deletion of exon 1 and approximately 200 base pairs of intron 1. Combined SKY-FISH analysis localized the integration site to Chromosome 3. GFP is expressed in adrenal glands of transgenic fetuses from about day E13 and in fetal renal arteries from about E14 through birth; it is also expressed in fetal adrenal and gonadal arteries. After birth, expression in renal arteries becomes progressively restricted, so that only juxtaglomerular cells fluoresce by approximately 12 weeks of age. Larger adult renal arteries can be induced to resume GFP expression by treatment with the ACE inhibitor captopril. Low levels of GFP expression occur in submandibular glands of male transgenic mice. (J:99617, J:99618)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:99618 Pan L, et al., Renin regulated GFP expression from a BAC transgene. MGI Direct Data Submission. 2005;
All:	3 reference(s)