Probe:	BR11
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		1C	There is ubiquitous expression throughout the endodermal, ectodermal, and mesodermal layers of the embryo.
TS13: foregut diverticulum	Present	Regionally restricted	1C	Expression is most intense in the anterior rim of the foregut, which is the primorida of the lung bud.

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		1C	There is no signal in the maternal tissue surrounding the conceptus.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		1F	Expression is ubiquitous. Low levels of expression were observed in all ectodermal, endodermal, and mesodermal derivatives.
TS15: heart	Weak		1F

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Weak		1I	Expression is the early central nervous system is either absent or expressed at low levels.
TS19: embryo mesenchyme	Strong	Regionally restricted	1I	Much of the connective tissue showed high levels of expression.
TS19: trunk somite	Strong		1I	Signficant levels of expression are found in the somites.
TS19: heart	Weak		1I
TS19: liver	Absent		1I
TS19: surface ectoderm	Strong		1I

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Weak		2C	Expression is the early central nervous system is either absent or expressed at low levels.
TS21: spinal cord	Present	Regionally restricted	2C	Expression is present in the ventral region of the developing spinal cord, including in the mantle layer.
TS21: spinal cord ventricular layer	Present		2C
TS21: hypothalamus	Weak		2C
TS21: thalamus	Weak		2C
TS21: telencephalon	Present	Regionally restricted	2C	There is expression in the hippocampus primordium.
TS21: hindbrain	Present	Regionally restricted	2C	Expression is present in the proliferating stem cell population within the ventricular zone. Lower levels of expression are also detected in postmitotic neurons within the mantle of the hindbrain.
TS21: midbrain	Present	Regionally restricted	2C	Expression is present in the most cranial aspect of the midbrain; the highest levels of expression are in the ventricular zone.
TS21: midbrain ventricular layer	Present	Regionally restricted	2C	Expression is present in the most cranial aspect of the midbrain; the highest levels of expression are in the ventricular zone.
TS21: cerebellum ventricular layer	Present		2C
TS21: cartilage	Strong	Regionally restricted	2C	There is strong expression in the cartilaginous primordia throughout the head region, including the primordia of the cranium and the nasal structures.
TS21: cranium	Strong	Regionally restricted	2C	There is strong expression in the cartilaginous primordia throughout the head region, including the primordia of the cranium and the nasal structures.
TS21: naris	Strong	Regionally restricted	2C	There is strong expression in the cartilaginous primordia throughout the head region, including the primordia of the cranium and the nasal structures.
TS21: limb cartilage condensation	Strong		2C
TS21: mandible	Strong		2C
TS21: maxilla	Strong		2C
TS21: vertebral cartilage condensation	Strong		2C
TS21: liver	Absent		2C
TS21: mesenchymal layer of primitive bladder	Weak		2C	Expression is present in the mesenchyme of the bladder.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong	Regionally restricted	3F , 3C	Expression is present in a tight band at the outer edge of the preplate.

Structure	Level	Pattern	Image	Note
TS25: brain	Present		4C	Expression is present at significant levels in the majority of neural structures within the brain.
TS25: cerebral cortex	Present	Regionally restricted	4C	Expression is present in the upper cortical layers. It is also present in the ependymal layer, adjacent to the lateral ventricles.
TS25: olfactory lobe	Present		4C	Expression is present in the olfactory bulb.
TS25: hippocampus	Strong		4C
TS25: mandible	Strong		4C
TS25: turbinate bone	Strong		4C
TS25: cerebellum	Present	Non-Uniform	4C	Expression is present at low levels throughout the central region, while no significant expression was observed in the cerebellar granular layer.
TS25: cranium	Strong	Regionally restricted	4C	There is intense expression in the cartilage of the cranium.
TS25: naris	Present	Regionally restricted	4C	Expression is present in the connective tissue surrounding the nasal epithelium but is not present in the nasal epithelium.
TS25: nasal cavity epithelium	Absent		4C

Structure	Level	Pattern	Image	Note
TS26: brain	Present		4F	Expression is present at significant levels in the majority of neural structures within the brain.
TS26: cerebral cortex	Present	Regionally restricted	4F	Expression is present in the upper cortical layers. It is also present in the ependymal layer, adjacent to the lateral ventricles.
TS26: cerebellum	Present	Non-Uniform	4F	Expression is present at low levels throughout the central region, while no significant expression was observed in the cerebellar granular layer.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Non-Uniform	4I	Expression is present at low levels throughout the central region, while no significant expression was observed in the cerebellar granular layer.

Structure	Level	Pattern	Image	Note
TS26: olfactory lobe	Present	Regionally restricted	5C	Expression is present in the olfactory bulb in the proliferating ependymal layer, a subset of the presumptive granular cell layer, and the outer mitrial cell layer.
TS26: cranium	Strong	Regionally restricted	5C	There is intense expression in the cartilage of the cranium.
TS26: mandible	Strong		5C
TS26: turbinate bone	Strong		5C
TS26: lower jaw molar	Strong		5C	Expression is present in the primordia.
TS26: upper jaw incisor	Strong		5C	Expression is present in the primordia.
TS26: upper jaw molar	Strong		5C	Expression is present in the primordia.
TS26: naris	Present	Regionally restricted	5C	Expression is present in the connective tissue surrounding the nasal epithelium but is not present in the nasal epithelium.
TS26: nasal cavity epithelium	Absent		5C

Structure	Level	Pattern	Image	Note
TS26: olfactory lobe	Present	Regionally restricted	5F	Expression is present in the olfactory bulb in the proliferating ependymal layer, a subset of the presumptive granular cell layer, and the outer mitrial cell layer.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	5I	Expression is present in the upper cortical layers.
TS26: hypothalamus	Present		5I
TS26: thalamus	Present		5I

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	5L	Expression is present in the ependymal layer, adjacent to the lateral ventricles.
TS26: 3rd ventricle	Strong	Regionally restricted	5L	Expression is present in the ependymal lining of the third ventricle.
TS26: hippocampus	Strong	Non-Uniform	5L	There is stronger expression in the CA2 and CA3 regions compared to the CA1 and CA4 areas.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	8B	Expression is present in the mesenchyme of the gut and its epithelium.
TS20: stomach epithelium	Present		8B
TS20: stomach mesenchyme	Present		8B

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Absent		8D	Expression in the stomach epithelium is negligible.
TS24: stomach mesenchyme	Present		8D

Structure	Level	Pattern	Image	Note
TS19: lung epithelium	Present		8F
TS19: lung mesenchyme	Present		8F

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		8H	Expression levels are higher in the mesenchyme than they are in the epithelium.
TS23: lung mesenchyme	Present		8H	Expression levels are higher in the mesenchyme than they are in the epithelium.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present			Expression is ubiquitous. Low levels of expression were observed in all ectodermal, endodermal, and mesodermal derivatives.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted		Expression is present in the ventral spinal cord. It is most intense in the ventricular layer, but it is also present in the mantle layer.
TS19: neural tube mantle layer	Present
TS19: neural tube ventricular layer	Present

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present
TS20: peripheral nervous system	Absent

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Strong
TS21: metanephros	Absent

Structure	Level	Pattern	Image	Note
TS22: tooth	Strong			There is strong expression in the tooth primordia.
TS22: renal medulla	Present	Regionally restricted		Expression is present in the medullary region of the kidney.
TS22: renal cortex	Present			Expression levels are lower in the cortex than in the medulla.
TS22: naris	Present	Regionally restricted		Expression is present in the connective tissue surrounding the nasal epithelium but is not present in the nasal epithelium.
TS22: nasal cavity epithelium	Absent

Structure	Level	Pattern	Image	Note
TS23: reproductive system	Strong	Regionally restricted		There is expression in the urogenital region, with the strongest expression at the tip of the genital tubercle.
TS23: naris	Present	Regionally restricted		Expression is present in the connective tissue surrounding the nasal epithelium but is not present in the nasal epithelium.
TS23: nasal cavity epithelium	Absent

Structure	Level	Pattern	Image	Note
TS24: reproductive system	Strong	Regionally restricted		There is expression in the urogenital region, with the strongest expression at the tip of the genital tubercle.
TS24: bladder	Present	Regionally restricted		There is expression in the mesenchyme surrounding the epithelium of the bladder but not in the epithelial layer.
TS24: sternum	Strong
TS24: heart	Weak
TS24: naris	Present	Regionally restricted		Expression is present in the connective tissue surrounding the nasal epithelium but is not present in the nasal epithelium.
TS24: nasal cavity epithelium	Absent
TS24: gut	Present	Regionally restricted		Expression is present in the mesenchyme, but not the epithelium, of the gut.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Weak
TS26: trigeminal ganglion	Present
TS26: peripheral nervous system	Absent