Gcat RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:98495 Edgar AJ, Mice have a transcribed L-threonine aldolase/GLY1 gene, but the human GLY1 gene is a non-processed pseudogene. BMC Genomics. 2005 Mar 9;6(1):32
Assay type:	RT-PCR
MGI Accession ID:	MGI:3582327
Gene symbol:	Gcat
Gene name:	glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)

Probe:	KBL-F; KBL-R
Assay notes:	This assay is a real time PCR.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	150 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
7-day embryo	E7.0 (a)	TS10: embryo	Weak	Not Specified; poly-A+ RNA	Not Specified		Not Specified
11-day embryo	E11.0 (a)	TS18: embryo	Present	Not Specified; poly-A+ RNA	Not Specified		Not Specified
15-day embryo	E15.0 (a)	TS23: embryo	Present	Not Specified; poly-A+ RNA	Not Specified		Not Specified
17-day embryo	E17.0 (a)	TS25: embryo	Present	Not Specified; poly-A+ RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was low at E7, but increased earlier than the other enzymes (see assays Tha1 MGI:3582324; Sds MGI:3582325; Tdh MGI:3582326).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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