Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64539
Hiltunen JO, et al., GDNF family receptors in the embryonic and postnatal rat heart and reduced cholinergic innervation in mice hearts lacking ret or GFRalpha2. Dev Dyn. 2000 Sep;219(1):28-39
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3581591
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Gene symbol:
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Slc18a3
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Gene name:
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solute carrier family 18 (vesicular monoamine), member 3
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Results
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Specimen
4D:
embryonic day 18.5
(more  )
Specimen
4D:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * MF1
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: heart |
Present |
Regionally restricted |
4D
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Expression is present in the cholinergic nerve fibers in the atrio-ventricular node, atrio-ventricular bundle, and proximal bundle branches.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129S/SvEv * MF1
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Mutant Allele(s):
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Rettm1Cos/Rettm1Cos
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: heart |
Present |
Regionally restricted |
4E
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In 2 of 6 mutant hearts there was an almost a complete absence of cholinergic nerve fibers in the atrio-ventricular node, atrio-ventricular bundle, and proximal bundle branches.
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Specimen
Clear reduction:
(close )
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Genetic Background:
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involves: 129S/SvEv * MF1
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Mutant Allele(s):
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Rettm1Cos/Rettm1Cos
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: heart |
Present |
Regionally restricted |
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In 3 of 6 mutant hearts there was a clear reduction in the number of cholinergic nerve fibers in the atrio-ventricular node, atrio-ventricular bundle, and proximal bundle branches.
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Specimen
No reduction:
(close )
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Genetic Background:
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involves: 129S/SvEv * MF1
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Mutant Allele(s):
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Rettm1Cos/Rettm1Cos
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: heart |
Present |
Regionally restricted |
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In 1 mutant heart (of 6) there was no reduction in the number of cholinergic nerve fibers in the atrio-ventricular node, atrio-ventricular bundle, and proximal bundle branches.
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Specimen
6A:
postnatal week 14-16
(more )
Specimen
6A:
(close )
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Genetic Background:
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involves: 129/OlaHsd * 129/SvHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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postnatal week 14-16
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: heart right ventricle |
Present |
Regionally restricted |
6A
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Expression is present in the cholinergic nerves, including those in the right ventricle.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129/OlaHsd * 129/SvHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Gfra2tm1Msa/Gfra2tm1Msa
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Age:
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postnatal week 14-16
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: heart right ventricle |
Present |
Regionally restricted |
6B
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Expression is present in the cholinergic nerves, but in the mutant their numbers are reduced 39% in the right ventricle.
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Specimen
6G:
postnatal week 14-16
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: 129/OlaHsd * 129/SvHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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postnatal week 14-16
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: impulse conducting system |
Present |
Regionally restricted |
6G
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Expression is present in the cholinergic nerves, including those in the atrio-ventricular node.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129/OlaHsd * 129/SvHsd * 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Gfra2tm1Msa/Gfra2tm1Msa
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Age:
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postnatal week 14-16
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: impulse conducting system |
Present |
Regionally restricted |
6H
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Expression is present in the cholinergic nerves, but in the mutant their numbers are reduced 59% in the atrio-ventricular node.
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Analysis Tools