Antibody:	anti-ADAM10
Detection system:	Secondary antibody
Assay notes:	The images were confocal images.

Structure	Level	Pattern	Image	Note
TS16: pancreas primordium	Present		1J , 1D	Expression was detected in the dorsal and ventral pancreatic buds as well as in the intervening gut endoderm. Expression was absent or near absent in the pancreas-associated mesenchyme and in a substantial number of cells that coexpressed glucagon.

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium	Weak	Regionally restricted		Expression is low or near absent in the glucagon expressing pancreatic epithelium; it is absent or near absent in the pancreas-associated mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Weak	Regionally restricted	2J	Expression was low or near absent in the pancreatic endocrine compartment.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Weak	Regionally restricted	2K	Expression was low or near absent in the pancreatic endocrine compartment.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	2L	Expression was prominent in glucagon-expressing cells, indicating an up-regulation in the endocrine compartment.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Weak	Regionally restricted	2M	Expression was low or near absent in the pancreatic endocrine compartment.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Weak	Regionally restricted	2N	Expression was low or near absent in the pancreatic endocrine compartment.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Regionally restricted	2O	Expression was prominent in insulin-expressing cells, indicating an up-regulation in the endocrine compartment.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Weak	Regionally restricted		Expression was low or near absent in the pancreatic endocrine compartment.

Structure	Level	Pattern	Image	Note
TS24: pancreas	Weak	Regionally restricted		Expression was low or near absent in the pancreatic endocrine compartment.

Structure	Level	Pattern	Image	Note
TS27: pancreatic islet core	Present		3D	Expression was detected in the beta cells.
TS27: pancreatic islet mantle	Present		3D	Expression was detected in the alpha and delta cells.
TS27: pancreas	Present	Regionally restricted	3D	Expression was prominent in exocrine pancreas as well as in alpha, beta and delta cells.

Structure	Level	Pattern	Image	Note
TS27: pancreatic islet core	Present		3E	Expression was detected in beta cells.
TS27: pancreatic islet mantle	Present		3E	Expression was detected in alpha and delta cells.
TS27: pancreas	Present	Regionally restricted	3E	Expression was prominent in exocrine pancreas as well as in alpha, beta and delta cells.

Structure	Level	Pattern	Image	Note
TS27: pancreatic islet core	Present		3F	Expression was detected in beta cells.
TS27: pancreatic islet mantle	Present		3F	Expression was detected in alpha and delta cells.
TS27: pancreas	Present	Regionally restricted	3F	Expression was prominent in exocrine pancreas as well as in alpha, beta and delta cells.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Regionally restricted	4G	Expression was detected throughout the epithelium.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present		5F , 5D	Expression was predominantly localized to the outer cell membrane in both the endocrine and exocrine compartment. Expression colocalized with Cdh1 (E-cadherin).

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	6D , 6F	Expression was detected in pancreatic ductal cells.

Structure	Level	Pattern	Image	Note
TS28: pancreatic duct	Present	Regionally restricted	6O , 6M	Expression was detected in the ductal epithelium.