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Tg(Per1-luc)025Jt
Transgene Detail
Summary
Symbol: Tg(Per1-luc)025Jt
Name: transgene insertion 025, Joseph S Takahashi
MGI ID: MGI:3578413
Synonyms: mPer1-luc, mPer1::luciferase, P1L025
Transgene: Tg(Per1-luc)025Jt  Location: unknown  
Alliance: Tg(Per1-luc)025Jt page
Transgene
origin
Strain of Origin:  CD-1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsA 6,756-base pair DNA fragment containing the putative promoter sequence and the 5'-untranslated region, which encompasses exon 1 and part of exon 2, was ligated to the luciferase gene so that the initiating ATGs of the Per1 and luc genes coincided. (J:73700)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
6 transgenic lines were generated and designated by the authors P1L005 (2 copies/genome), P1L025 (1 copy), P1L095 (3), P1L097 (1), P1L128 (2) and P1L141 (10). Brain sections of mice of line Tg(Per1-luc)025Jt (P1L025) were hybridized with luc and Per1 probes to verify the ability of the promoter to maintain a normal circadian pattern of expression after seven days in constant darkness. As results of other experiments were similar for all six lines, data were not differentiated by line.

Mice hemizygous for the transgene express luc mRNA in the suprachiasmatic nuclei (SCN) with a circadian rhythm. SCN slice cultures exhibit a circadian rhythm of luminescence for at least 5 days, peaking each day at 6 hours circadian time. Exposure of mice to light for 6 hours during early subjective night rapidly induces transcription of both luc and endogenous Per1 in the SCN; however, whereas Per1 mRNA levels return to baseline, luc mRNA levels remain high.
References
Original:  J:73700 Wilsbacher LD, et al., Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice invivo. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):489-94
All:  4 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory