Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:91734
Wang Y, et al., Entire mitogen activated protein kinase (MAPK) pathway is present in preimplantation mouse embryos. Dev Dyn. 2004 Sep;231(1):72-87
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3578379
|
|
Gene symbol:
|
Mapk1
|
|
Gene name:
|
mitogen-activated protein kinase 1
|
|
Antibody:
|
anti-p44/42 MAP Kinase (#9102)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
|
|
Assay notes:
|
The authors did not specify which antibody was used. They used either ERK1/2 (SC-94) (MGI:3576754), or p44/42 MAP Kinase (9102) (MGI:3577737).
|
|
|
Results
|
Specimen
10D,F:
embryonic day 3.5
(more  )
Note: Morula/early cavitation blastocyst.
Specimen
10D,F:
(close  )
|
Genetic Background:
|
involves: C57BL/6J * MF1 * SJL/J
|
|
Age:
|
embryonic day 3.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Note:
|
Morula/early cavitation blastocyst.
|
Specimen
11D,F:
embryonic day 2.5
(more )
Specimen
11D,F:
(close )
|
Genetic Background:
|
involves: C57BL/6J * MF1 * SJL/J
|
|
Age:
|
embryonic day 2.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
2% Paraformaldehyde
|
|
Analysis Tools
