Probe:	Dac cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from head mesoderm	Present		2A
TS15: optic cup	Present		2A
TS15: otocyst	Present		2A
TS15: telencephalon	Present		2A
TS15: trunk mesenchyme	Present		2A
TS15: neural tube	Present	Regionally restricted	2A	Expression is dorsal.
TS15: future midbrain	Absent		2A

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present	Regionally restricted	2B	Expression is in the neocortex.
TS18: head mesenchyme	Present	Regionally restricted	2B	Expression is in the mesenchyme surrounding the eye.
TS18: retina	Present	Regionally restricted	2B	Expression is in the neuroretina adjacent to the lens.
TS18: dorsal root ganglion	Present		2B
TS18: limb	Present		2B
TS18: trigeminal ganglion	Present		2B
TS18: 2nd branchial arch	Present	Regionally restricted	2B	Expression is at the boundary between arch 2 and 3.
TS18: 3rd branchial arch	Present	Regionally restricted	2B	Expression is at the boundary between arch 2 and 3.
TS18: future spinal cord	Strong	Regionally restricted	2B	Expression is dorsal.
TS18: hindbrain	Strong	Regionally restricted	2B	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong		2C
TS20: limb	Strong		2C
TS20: spinal cord	Weak		2C
TS20: tail mesenchyme derived from neural crest	Weak		2C
TS20: corpus striatum	Absent		2C

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong		2D
TS21: genital tubercle	Strong		2D
TS21: limb	Strong		2D

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong		2E
TS21: genital tubercle	Strong		2E
TS21: limb	Strong		2E

Structure	Level	Pattern	Image	Note
TS13: optic pit	Present		3A
TS13: future prosencephalon	Present	Regionally restricted	3A	Expression is in a band of cells at the anterior rim of the prosencephalon and in the lamina terminalis.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	3B	Expression is in a band of cells at the anterior rim of the prosencephalon and in the lamina terminalis.
TS13: 1st branchial arch	Present		3B
TS13: 2nd branchial arch	Present		3B

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		3C
TS13: head mesenchyme	Present		3C
TS13: optic pit	Present		3C
TS13: future midbrain	Absent		3C
TS13: future hindbrain	Absent		3C	Absent in the metencephalon.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		3D
TS13: head mesenchyme	Present		3D
TS13: optic pit	Present		3D
TS13: future midbrain	Absent		3D
TS13: future hindbrain	Absent		3D	Absent in the metencephalon.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	3E	Expression is dorsal.
TS14: optic vesicle	Present		3E

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	3F	Expression is dorsal.
TS14: optic vesicle	Present		3F

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	3G	Expression is in two dorsal areas separated by the midline which is absent in expression.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	3H	Expression is in two dorsal areas separated by the midline which is absent in expression.
TS15: diencephalon lamina terminalis	Weak		3H

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present	Regionally restricted	3I	Expresion is in two lateral fields of cranial and trunk mesenchymal cells that stretch anteriorly to the margin of the foregut pouch where they split into two areas.
TS12: trunk mesenchyme	Present	Regionally restricted	3I	Expresion is in two lateral fields of cranial and trunk mesenchymal cells that stretch anteriorly to the margin of the foregut pouch where they split into two areas.
TS12: 1st branchial arch mesenchyme	Present		3I

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	3J	Expression is dorsal.
TS13: future spinal cord neural crest	Present		3J
TS13: head mesenchyme	Present		3J
TS13: lateral plate mesoderm	Present		3J
TS13: trunk mesenchyme	Present		3J

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		3K
TS15: future spinal cord	Present	Regionally restricted	3K	Expression is dorsal.
TS15: future midbrain	Absent		3K

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	3L	Expression is dorsal.
TS15: trunk mesenchyme derived from neural crest	Present		3L

Structure	Level	Pattern	Image	Note
TS18: neural tube	Strong	Regionally restricted	3M	Expression is dorsal. Position is at the hindlimb.
TS18: migrating neural crest	Present	Regionally restricted	3M	Expression is in migrating neural crest.

Structure	Level	Pattern	Image	Note
TS18: neural tube	Strong	Regionally restricted	3N	Expression is dorsal.
TS18: dorsal root ganglion	Strong		3N
TS18: sympathetic ganglion	Strong		3N

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present	Regionally restricted	3O	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	3Q	Expression is in the dorsal rim.

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Present	Regionally restricted	3R	Expression is in the head mesenchyme surrounding the eye.
TS18: retina	Present		3R
TS18: trigeminal ganglion	Present		3R
TS18: 2nd branchial arch	Present	Regionally restricted	3R	Expression is between arches 2 and 3.
TS18: 3rd branchial arch	Present	Regionally restricted	3R	Expression is between arches 2 and 3.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 3	Present	Regionally restricted	3S	Expression is ventral in two groups.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		4A
TS15: otocyst	Present		4A

Structure	Level	Pattern	Image	Note
TS18: otocyst	Present	Regionally restricted	4B	Expression is in dorsolateral areas.

Structure	Level	Pattern	Image	Note
TS20: interdigital region between forelimb digits 1 and 2	Present		4C
TS20: interdigital region between forelimb digits 2 and 3	Present		4C
TS20: forelimb digit 2 mesenchyme	Absent		4C	Absent in the chondral condensations.
TS20: forelimb digit 2 epithelium	Absent		4C

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present	Regionally restricted	4D	Expression is in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	4E	Expression is dorsal.
TS15: trunk mesenchyme	Strong	Regionally restricted	4E	Expression is in the lateral mesenchyme.

Structure	Level	Pattern	Image	Note
TS18: dorsal root ganglion	Strong		4F

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present		4G
TS15: telencephalon	Present	Regionally restricted	4G	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	4H	Expression is in the distal portion.
TS15: optic stalk	Absent		4H

Structure	Level	Pattern	Image	Note
TS20: retina	Present		4I

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	4K	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Strong	Regionally restricted	4L	Expression is in the lens ectoderm.
TS15: diencephalon lamina terminalis	Strong		4L

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		4M

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	5B	Expression is in three areas of the mesenchyme. An anterior area of expression that extends from the anterior margin of the limb bud deep towards the midline, a posterior band, and a faint zone proximal to the ZPA.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	5C	Expression is in three area sof the mesenchyme. An anterior area of expression that extends from the anterior margin of the limb bud deep towards the midline, a posterior band, and a faint zone proximal to the ZPA.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		5D

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		5E

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 1	Present	Regionally restricted	5F	Expression is at the tip.
TS20: forelimb digit 2	Present	Regionally restricted	5F	Expression is at the tip.
TS20: forelimb digit 3	Present	Regionally restricted	5F	Expression is at the tip.
TS20: forelimb digit 4	Present	Regionally restricted	5F	Expression is at the tip.
TS20: forelimb digit 5	Present	Regionally restricted	5F	Expression is at the tip.
TS20: interdigital region between forelimb digits 1 and 2	Present		5F
TS20: interdigital region between forelimb digits 2 and 3	Present		5F
TS20: interdigital region between forelimb digits 3 and 4	Present		5F
TS20: interdigital region between forelimb digits 4 and 5	Present		5F

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 1	Strong	Regionally restricted	5G	Expression is at the tip.
TS21: forelimb digit 2	Strong	Regionally restricted	5G	Expression is at the tip.
TS21: forelimb digit 3	Strong	Regionally restricted	5G	Expression is at the tip.
TS21: forelimb digit 4	Strong	Regionally restricted	5G	Expression is at the tip.
TS21: forelimb digit 5	Strong	Regionally restricted	5G	Expression is at the tip.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		6A
TS19: hindlimb bud mesenchyme	Present		6A

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6B

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6C

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6D	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6E	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Strong	Regionally restricted	6F	Expression is increased in the anterior mesenchyme compared to wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Strong	Regionally restricted	6G	Expression is increased in the anterior mesenchyme compared to wild-type embryos.