Antibody:	anti-alpha7 integrin
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The fixative was 0.2% paraformaldehyde in 0.1M phosphate buffer with 0.12mM CaCl2, 4% sucrose.

Structure	Level	Pattern	Image	Note
TS19: trunk dermomyotome	Present	Regionally restricted	1B	Expression was detected only in the elongated myotome cells.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Absent		1J	Immunoreactivity was absent from the forelimb myogenic cells.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Absent			Immunoreactivity was absent from muscle masses.
TS20: trunk mesenchyme	Absent			Immunoreactivity was absent from muscle masses.

Structure	Level	Pattern	Image	Note
TS21: pectoral girdle and thoracic body wall muscle	Present	Regionally restricted	2J	Expression was detected in trunk muscles and was most evident in the intercostal, pectoral and deep back muscle cells.
TS21: vertebral axis musculature	Present	Regionally restricted	2J	Expression was detected in trunk muscles and was most evident in the intercostal, pectoral and deep back muscle cells.