Antibody:	Anti-laminin alpha2-chain
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The fixative was 0.2% paraformaldehyde in 0.1M phosphate buffer with 0.12mM CaCl2, 4% sucrose.

Structure	Level	Pattern	Image	Note
TS19: trunk dermomyotome	Present	Regionally restricted	1C	Expression was detected around myotomal cells and was enriched at myotomal borders.

Structure	Level	Pattern	Image	Note
TS19: trunk dermomyotome	Present	Regionally restricted	1C Inset	Expression was enriched at myotomal borders.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Absent		1K	Immunoreactivity was absent from forelimb myogenic cells.

Structure	Level	Pattern	Image	Note
TS20: body wall mesenchyme	Absent		2C	Immunostaining was not detected in abdominal wall muscle masses.

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	2K	Expression was detected in trunk muscles.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Absent		3C	Immunoreactivity was absent from forelimb muscle masses.