Antibody:	2A1
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Antibody recognizes both isoforms, Gpm6a and Gpm6b.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	1a	Expression is in cells of the preplate, absent in the ventricular zone.
TS20: choroid plexus	Present	Regionally restricted	1a	Expression is in epithelial cells.
TS20: blood vessel	Absent		1a	Shown is the brain.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	1b	Expression is in cell bodies in the preplate and the subplate and in axons in the subplate and intermediate zone.
TS22: cerebral cortex marginal layer	Present		1b
TS22: cerebral cortex intermediate zone	Present		1b	Expression is in cell bodies in the preplate and the subplate and in axons in the subplate and intermediate zone.
TS22: choroid plexus	Very strong	Regionally restricted	1b	Expression is in epithelial cells.
TS22: blood vessel	Absent		1b	Shown is the brain.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	1c , 1e	Expression is in cell bodies in the preplate. Absent in the ventricular zone.
TS24: choroid plexus	Present	Regionally restricted	1e , 1c	Expression is in epithelial cells.
TS24: blood vessel	Absent		1c , 1e	Shown is the brain.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Weak	Regionally restricted	1d	Weak in the cortex but absent in the ventricular zone.
TS26: choroid plexus	Present	Regionally restricted	1d	Expression is in epithelial cells.
TS26: telencephalon	Present	Regionally restricted	1d	Expression is in the subcortical white matter.
TS26: blood vessel	Absent		1d	Shown is the brain.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak	Uniform
TS28: cerebral hemisphere white matter	Absent
TS28: corpus callosum	Absent

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	2_E12	Labeling is in the preplate.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2_E14	Expression is in the intermediate zone and cortical plate, absent in the ventricular zone.
TS22: cerebral cortex intermediate zone	Present		2_E14
TS22: cortical plate	Present		2_E14

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	2_E16	Expression is in the intermediate zone and cortical plate, absent in the ventricular zone.
TS24: cerebral cortex intermediate zone	Present		2_E16
TS24: cortical plate	Present		2_E16

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	2_E19	Expression is in the intermediate zone and cortical plate, absent in the ventricular zone.
TS26: cerebral cortex intermediate zone	Present		2_E19
TS26: cortical plate	Present		2_E19

Structure	Level	Pattern	Image	Note
TS21: spinal cord mantle layer	Present		3a , 3b
TS21: spinal cord marginal layer	Present		3a , 3b
TS21: spinal cord ventricular layer	Present	Regionally restricted	3a , 3b	Expression is absent in the proliferative zone surrounding the central canal.
TS21: dorsal root ganglion	Absent		3a , 3b
TS21: skeletal muscle	Absent		3a , 3b

Structure	Level	Pattern	Image	Note
TS21: early nephron	Present	Regionally restricted		Expression is in the luminal surface of the epithelium of the proximal tubule.
TS21: cartilage	Absent
TS21: heart	Absent
TS21: intestine	Absent
TS21: liver	Absent
TS21: skin	Absent

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		4a
TS23: cortical plate	Present		4a
TS23: choroid plexus	Strong		4a