Probe:	IMAGE clone 1195364
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Spotted		Positive cells were scattered throughout the embryo.

Structure	Level	Pattern	Image	Note
TS20: cardiovascular system	Present	Regionally restricted	4A	Expression is in the endothelial cells of the vascular system.

Structure	Level	Pattern	Image	Note
TS20: cardiovascular system	Present	Regionally restricted	4B	Expression is in the endothelial cells of the vascular system. Shown are capillaries in the olfactory epithelium and the arachnoid.

Structure	Level	Pattern	Image	Note
TS20: cardiovascular system	Present	Regionally restricted	4D	Expression is in the endothelial cells of the vascular system. Shown are capillaries in the tail.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		4F

Structure	Level	Pattern	Image	Note
TS20: cranial nerve	Present
TS20: spinal ganglion	Present
TS20: brain	Absent

Structure	Level	Pattern	Image	Note
TS22: cardiovascular system	Present	Regionally restricted	5A	Expression is in the endothelial cells of the vascular system.
TS22: dorsal root ganglion	Present		5A
TS22: metanephros	Strong		5A

Structure	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	Strong		5B

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	5D	Expression is in a layer of cells below the leptomeninges.

Structure	Level	Pattern	Image	Note
TS22: brain	Present
TS22: corpus striatum	Present

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Graded	5F	High levels were visible in the rostral part and the hippocampal area of the cortical plate. The rest of the cortical plate showed a low level of expression.
TS24: cardiovascular system	Present	Regionally restricted	5F	Expression is in the endothelial cells of the vascular system.
TS24: corpus striatum	Present		5F
TS24: cranial nerve	Present		5F
TS24: maturing glomerular tuft	Present		5F
TS24: spinal component of peripheral nervous system	Present		5F

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	5G	Expression is in the external granular layer.

Structure	Level	Pattern	Image	Note
TS24: cardiovascular system	Trace	Regionally restricted	5I	Expression is in the endothelial cells. Shown are the mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS26: cardiovascular system	Present	Regionally restricted	5K	Expression is in the endothelial cells.
TS26: lung	Trace	Regionally restricted	5K	Expression is in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS26: cardiovascular system	Present	Regionally restricted	5L
TS26: corpus striatum	Strong		5L
TS26: cranial ganglion	Strong		5L
TS26: spinal ganglion	Strong		5L
TS26: cortical plate	Strong		5L
TS26: cerebellum	Strong	Regionally restricted	5L	Expression is in the external granular layer.
TS26: prethalamus	Strong		5L	Expression is in the subthalamic nucleus.