Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74049
Segat D, et al., Expression of matrilin-1, -2 and -3 in developing mouse limbs and heart. Matrix Biol. 2000 Dec;19(7):649-55
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3576359
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Gene symbol:
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Matn1
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Gene name:
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matrilin 1, cartilage matrix protein
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Antibody:
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anti-matrilin 1
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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The tissue was frozen, sectioned, and then fixed with 1% paraformaldehyde in PBS for 10 minutes.
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Results
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Specimen
1 9.5 dpc:
embryonic day 10.0
(more  )
Specimen
1 9.5 dpc:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
1 10.5 dpc:
embryonic day 11.0
(more )
Specimen
1 10.5 dpc:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: heart |
Present |
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1_10.5 dpc Matn1
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Specimen
E14.5--not shown:
embryonic day 15.0
(more )
Specimen
E14.5--not shown:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 15.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3 12.5 dpc:
embryonic day 13.0
(more )
Specimen
3 12.5 dpc:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 13.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
E12.5--not shown:
embryonic day 13.0
(more )
Specimen
E12.5--not shown:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 13.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3 13.5 dpc:
embryonic day 14.0
(more )
Specimen
3 13.5 dpc:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 14.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3 14.5 dpc left:
embryonic day 15.0
(more )
Specimen
3 14.5 dpc left:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 15.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forelimb skeleton |
Present |
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3_14.5 dpc Matn1 left
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Expression is present in the cartilage.
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| TS23: hand mesenchyme |
Present |
Regionally restricted |
3_14.5 dpc Matn1 left
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Expression is present in the tendon at its insertion into the third phalange.
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Specimen
3 14.5 dpc right:
embryonic day 15.0
(more )
Specimen
3 14.5 dpc right:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 15.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forelimb skeleton |
Present |
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3_14.5 dpc Matn1 right
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Expression is present in the cartilage.
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Specimen
4:
embryonic day 15.0
(more )
Specimen
4:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 15.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: humerus |
Present |
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4_Matn1
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Expression is present in the elbow, in the zones of proliferating and early hypertrophic chondrocytes.
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| TS23: ulna |
Present |
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4_Matn1
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Expression is present in the elbow, in the zones of proliferating and early hypertrophic chondrocytes.
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Specimen
5 15.5 dpc:
embryonic day 16.0
(more )
Specimen
5 15.5 dpc:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 16.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: forelimb skeleton |
Present |
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5_15.5 dpc Matn1
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Expression is present in cartilage of the developing bone anlage.
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| TS24: hand mesenchyme |
Present |
Regionally restricted |
5_15.5 dpc Matn1
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Expression is present in the ligaments and tendons about the carpus.
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| TS24: hand dermis |
Present |
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5_15.5 dpc Matn1
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Specimen
5 17.5 dpc left:
embryonic day 18.0
(more )
Specimen
5 17.5 dpc left:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forelimb skeleton |
Present |
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5_17.5 dpc Matn1 left
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Expression is present in cartilage of the developing bone anlage.
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| TS26: hand mesenchyme |
Present |
Regionally restricted |
5_17.5 dpc Matn1 left
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Expression is present in the ligaments and tendons about the carpus.
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| TS26: hand dermis |
Present |
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5_17.5 dpc Matn1 left
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Specimen
5 17.5 dpc right:
embryonic day 18.0
(more )
Specimen
5 17.5 dpc right:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forelimb skeleton |
Present |
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5_17.5 dpc Matn1 right
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Expression is present in cartilage of the developing bone anlage.
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| TS26: hand mesenchyme |
Present |
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5_17.5 dpc Matn1 right
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Expression is present in the ligaments and tendons about the carpus.
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| TS26: hand dermis |
Present |
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5_17.5 dpc Matn1 right
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Specimen
6:
embryonic day 16.0
(more )
Specimen
6:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 16.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Non-Uniform |
6_Matn1
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Expression is not as intensive at the articular surface as it is in the deeper zone of proliferating cells.
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| TS24: ulna |
Present |
Non-Uniform |
6_Matn1
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Expression is not as intensive at the articular surface as it is in the deeper zone of proliferating cells.
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Analysis Tools