Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89190
Poschl E, et al., Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development. Development. 2004 Apr;131(7):1619-28
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3576215
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Gene symbol:
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Col4a2
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Gene name:
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collagen, type IV, alpha 2
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Antibody:
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anti-collagen IV
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Detection system:
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Secondary antibody
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Assay notes:
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Authors did not detail which collagen IV family members are recognized by this antibody.
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Results
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Specimen
3A +/-:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Del(8Col4a1-Col4a2)1Epo/+
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
3A Col4 +/-
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Expression is present in basement membranes.
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Specimen
3A -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Del(8Col4a1-Col4a2)1Epo/Del(8Col4a1-Col4a2)1Epo
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3B +/+:
embryonic day 10.5
(more  )
Specimen
3B +/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3B -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Del(8Col4a1-Col4a2)1Epo/Del(8Col4a1-Col4a2)1Epo
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Analysis Tools