Gene Expression Data
Assay Details
Assay
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Reference:
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J:26073
Fowlis D, et al., Galectin-3 is expressed in the notochord, developing bones, and skin of the postimplantation mouse embryo. Dev Dyn. 1995 Jun;203(2):241-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3575722
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Gene symbol:
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Lgals3
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Gene name:
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lectin, galactose binding, soluble 3
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Probe:
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clone 1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A,B:
embryonic day 8.5
(more )
Specimen
1A,B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 8.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Specimen
Not Shown:
embryonic day 7.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Specimen
Not Shown:
embryonic day 7.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Specimen
2A,B:
embryonic day 8.5
(more )
Note: 9-12 somites.
Specimen
2A,B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 8.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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9-12 somites.
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Specimen
2E:
embryonic day 9.5
(more )
Note: 20-25 somites.
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 9.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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20-25 somites.
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Structure |
Level |
Pattern |
Image |
Note |
TS15: notochord |
Present |
Graded |
2E
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The strongest signal was around the 12th pair of somites.
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Specimen
Not Shown:
embryonic day 12.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Specimen
Not Shown:
embryonic day 13.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cartilage |
Strong |
|
|
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TS21: limb digit |
Weak |
Patchy |
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Expression is in the 1-cell deep suprabasal layer of the epidermis.
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TS21: head |
Weak |
Patchy |
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Expression is in the 1-cell deep suprabasal layer of the epidermis on the muzzle.
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Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3B,C:
embryonic day 14.5
(more )
Specimen
3B,C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3D,E:
embryonic day 14.5
(more )
Specimen
3D,E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: humerus cartilage condensation |
Strong |
Regionally restricted |
3D/E
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Moderate expression in cartilage and very high levels in the transition zone (hypertrophic chondrocytes). Absent in osteoblasts.
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Specimen
4A,B:
embryonic day 15.5
(more )
Specimen
4A,B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 15.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: epidermis |
Strong |
Regionally restricted |
4A/B
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Expression is in the suprabasal layers and in the central cells of the hair follicles.
|
TS23: dermis |
Absent |
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4A/B
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Specimen
Not Shown:
postnatal newborn
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal newborn
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: epidermis |
Present |
Regionally restricted |
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Expression is in the suprabasal layers.
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TS27: dermis |
Absent |
|
|
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Specimen
Not Shown:
postnatal adult
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: epidermis |
Present |
Regionally restricted |
|
Expression is in the basal and suprabasal layers.
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TS28: dermis |
Absent |
|
|
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TS28: hair follicle |
Present |
Regionally restricted |
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Expression is in the inner root sheath of hair follicles.
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Specimen
Not Shown:
embryonic day 11.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: liver |
Present |
Single cells |
|
|
Specimen
5A,B:
embryonic day 12.5
(more )
Specimen
5A,B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5C,D:
embryonic day 14.5
(more )
Specimen
5C,D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Specimen
5E,F:
embryonic day 14.5
(more )
Specimen
5E,F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: bladder |
Present |
Regionally restricted |
5E/F
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Expression is in the endodermal lining of the bladder.
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Specimen
5G,H:
embryonic day 14.5
(more )
Specimen
5G,H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
Not shown:
embryonic day 14.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
Not shown:
embryonic day 15.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: esophagus |
Present |
Regionally restricted |
|
Expression is in the lining.
|
TS23: liver |
Present |
Single cells |
|
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TS23: skeleton |
Present |
Single cells |
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Expression is in 2-3 cells infiltrating the mineralizing part of the bones.
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