Antibody:	anti-Neurod1 (N-19; sc-1084)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Fixation was in methanol/acetone.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Present	Regionally restricted	5B	A small number of cells in the dorsal region also label with Gata3. A large number of cells in the ventral region are positive and there is clear overlap with Gata3.
TS15: trigeminal preganglion	Present		5B

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Single cells	5D	Expression is in migrating neuroblasts along the anterior edge.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Absent		5F
TS19: inner ear	Present	Single cells	5F	Expression is in migrating neuroblasts.

Structure	Level	Pattern	Image	Note
TS21: vestibular ganglion	Present	Uniform	6B
TS21: cochlear ganglion	Present	Single cells	6B

Structure	Level	Pattern	Image	Note
TS13: otic placode	Absent
TS13: inner ear	Present	Single cells		Expression is in a few cells close to the ventral edge of the otic placode.