Probe:	cfm cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent			Absent in the 3 somite stage.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	1a	Expression is in the caudal forebrain.
TS12: future midbrain	Present		1a

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present	Regionally restricted	1b	Expression is in the caudal forebrain.
TS12: future midbrain	Present		1b
TS12: future spinal cord neural plate	Absent		1b	Absent in the rostral neural plate.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present		1c

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present		1d

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Weak		1e

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Weak		1f
TS13: future prosencephalon	Present	Regionally restricted	1f	Expression is in the caudal forebrain.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		1g	Expression is in the caudal forebrain.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Absent		1h	Absent in the rostral neural plate.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Weak	Regionally restricted	1i	Expression is in a broad stripe pattern in the midregion.
TS14: prosencephalon	Present	Regionally restricted	1i	Expression is in the caudal forebrain.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Weak	Regionally restricted	1j	Expression is in a broad stripe pattern in the midregion.
TS14: prosencephalon	Present	Regionally restricted	1j	Expression is in the caudal forebrain.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1k	Expression is in the dorsal thalamus.
TS15: future midbrain	Trace		1k
TS15: 1st branchial arch	Present	Regionally restricted	1k	Expression is in the rostral aspect.
TS15: Rathke's pouch	Present		1k

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1l	Expression is in the dorsal thalamus.
TS15: future midbrain	Trace		1l
TS15: 1st branchial arch	Present	Regionally restricted	1l	Expression is in the rostral aspect.
TS15: Rathke's pouch	Present		1l

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present	Regionally restricted	1m	Expression is in the epiphysis.
TS20: 3rd ventricle	Absent		1m
TS20: brain mantle layer	Absent		1m
TS20: brain ventricular layer	Absent		1m
TS20: choroid plexus	Absent		1m
TS20: diencephalon floor plate	Absent		1m
TS20: diencephalon gland	Absent		1m
TS20: diencephalon lamina terminalis	Absent		1m
TS20: diencephalon lateral wall	Absent		1m
TS20: diencephalon mantle layer	Absent		1m
TS20: diencephalon roof plate	Absent		1m
TS20: diencephalon ventricular layer	Absent		1m
TS20: hindbrain	Absent		1m
TS20: hypothalamus	Absent		1m
TS20: midbrain	Absent		1m
TS20: telencephalon	Absent		1m
TS20: thalamus	Absent		1m

Structure	Level	Pattern	Image	Note
TS20: epithalamus	Present	Regionally restricted	1n	Expression is in the epiphysis.
TS20: 3rd ventricle	Absent		1n
TS20: brain mantle layer	Absent		1n
TS20: brain ventricular layer	Absent		1n
TS20: choroid plexus	Absent		1n
TS20: diencephalon floor plate	Absent		1n
TS20: diencephalon gland	Absent		1n
TS20: diencephalon lamina terminalis	Absent		1n
TS20: diencephalon lateral wall	Absent		1n
TS20: diencephalon mantle layer	Absent		1n
TS20: diencephalon roof plate	Absent		1n
TS20: diencephalon ventricular layer	Absent		1n
TS20: hindbrain	Absent		1n
TS20: hypothalamus	Absent		1n
TS20: midbrain	Absent		1n
TS20: telencephalon	Absent		1n
TS20: thalamus	Absent		1n

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present		1o
TS20: lung	Present		1o
TS20: occipital myotome	Present		1o
TS20: peripheral nervous system	Present	Regionally restricted	1o	Expression is in peripheral nerves.
TS20: alimentary system	Present	Regionally restricted	1o	Expression is in the inner wall of the alimentary canal.