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Gene Expression Data
Assay Details
Assay
Reference: J:96322 Murphy K, et al., Expression of Stat3 in germ cells of developing and adult mouse ovaries and testes. Gene Expr Patterns. 2005 Apr;5(4):475-82
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3575102
Gene symbol: Stat3
Gene name: signal transducer and activator of transcription 3
Antibody: anti-Stat3 (C-20) sc-482
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A,C: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 1A , 1C
TS28: ovary follicle cell Absent 1C , 1A Expression is in in oocytes but not in granulosa or other somatic cells.
Specimen 1D: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: oocyte of primary follicle Present Single cells 1D Expression is detected in the cytoplasm of oocytes from primordial, primary and secondary follicles.
TS28: oocyte of primordial follicle Present Single cells 1D Expression is detected in the cytoplasm of oocytes from primordial, primary and secondary follicles.
TS28: oocyte of secondary follicle Present Single cells 1D Expression is detected in the cytoplasm of oocytes from primordial, primary and secondary follicles.
Specimen 1E: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present 1E Expression is detected in the cytoplasm of oocytes from secondary follicles.
Specimen 1F: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: ovary antral follicle Absent 1F Expression is absent in preantral or antral follicles.
TS28: ovary preantral follicle Absent 1F Expression is absent in preantral or antral follicles.
Specimen 1G,I: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: spermatid Present Single cells 1G , 1I Expression is in the developing acrosomes of round spermatids.
Specimen 1J: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: spermatid Present Single cells 1J Expression is in the developing acrosomes of round spermatids.
Specimen 1K: postnatal day 42 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Cell Type Level Pattern Image Note
TS28: spermatid Present Regionally restricted 1K Expression is in the developing acrosomes of round spermatids.
TS28: spermatocyte Absent 1K
TS28: spermatogonium Absent 1K
TS28: Sertoli cell Sertoli cell Absent 1K
Specimen 3A,C: embryonic day 12.5 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS20: ovary Absent 3C , 3A
Specimen 3D,F: embryonic day 13.5 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS21: germ cell of ovary Strong 3D , 3F
TS21: mesenchymal stroma of ovary Weak 3F , 3D
Specimen 3G,I: postnatal day 1 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS27: oocyte Strong 3G , 3I
TS27: ovary Present Regionally restricted 3I , 3G Weak expression in somatic cells.
Specimen 3J,L: postnatal day 4 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS28: oocyte Strong 3J , 3L
TS28: ovary follicle cell Absent 3J , 3L
Specimen 4A,C: embryonic day 12.5 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS20: testis Absent 4C , 4A
Specimen 4D,F: embryonic day 13.5 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS21: germ cell of testis Present Regionally restricted 4D , 4F
Specimen 4G,I: postnatal day 1 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Cell Type Level Pattern Image Note
TS27: germ cell of testis Present Regionally restricted 4I , 4G Expression is in prospermatogonia.
TS27: Sertoli cell Sertoli cell Weak 4G , 4I
Specimen 4J,L: postnatal day 4 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Cell Type Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 4L , 4J Expression is in prospermatogonia.
TS28: Sertoli cell Sertoli cell Weak 4L , 4J
Specimen 5B,C: embryonic day 13.5 (more )
Note: Fixative was 5% paraformaldehyde.
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 5C , 5B
Specimen 5F,G: postnatal day 1 (more )
Note: Fixative was 5% paraformaldehyde. Double labeled: red - Stat3; green - Gcna (assay MGI:3575274).
Structure Level Pattern Image Note
TS27: ovary Present 5F , 5G Staining overlapped with that of Gcna1.
Specimen 5J,K: postnatal day 1 (more )
Note: Fixative was 5% paraformaldehyde. Double labeled: red - Stat3; green - Gcna (assay MGI:3575274).
Structure Level Pattern Image Note
TS27: testis Present 5K , 5J Staining overlapped with that of Gcna1.
Specimen 5M,O: postnatal day 1 (more )
Note: Fixative was 5% paraformaldehyde. Double labeled: red - Stat3; green - Ddx4 (assay MGI:3575259).
Structure Level Pattern Image Note
TS27: ovary Present 5M , 5O Staining overlapped with that of Ddx4.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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