Probe:	IMAGE clone 1297376
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall ventricular layer	Present		2A
TS19: telencephalon ventricular layer	Present		2A

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2C , 2B	Expression is in germinal zones; the cortical ventricular zone and the ganglionic eminences.
TS21: ganglionic eminence	Present		2C , 2B
TS21: cortical plate	Present		2B , 2C	Lower levels in the cortical plate.

Structure	Level	Pattern	Image	Note
TS23: forebrain	Present	Regionally restricted	2D	Expression is in the proliferating regions of the forebrain in addition to the dorsal thalamus, cortical plate, the emerging habenular nucleus and the cortical hem of the developing hippocampus.
TS23: cortical plate	Present		2D

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	2F , 2G , 2E	Expression is in the cortical ventricular zone, cortical plate, and ganglionic eminences and in lower levels in the intermediate zone and marginal zone.
TS25: cortical plate	Present		2G , 2E , 2F
TS25: ganglionic eminence	Present		2G , 2E , 2F
TS25: cerebral cortex intermediate zone	Weak		2E , 2G , 2F

Structure	Level	Pattern	Image	Note
TS25: forebrain	Present	Regionally restricted	2H	Expression is in the anterior arm of the subventricular zone extending into the olfactory bulbs.
TS25: olfactory lobe	Present	Regionally restricted	2H	Expression is in the anterior arm of the subventricular zone extending into the olfactory bulbs.
TS25: midbrain	Absent		2H

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present	Regionally restricted		Expression is in the external granule layer.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Very strong	Regionally restricted	3A	Expression is in the anterior part of subventricular zone.
TS27: hippocampus	Present		3A
TS27: rostral migratory stream	Present		3A
TS27: neocortex	Weak		3A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Very strong		3B , 3C
TS27: brain subventricular zone	Very strong	Regionally restricted	3B , 3C	Expression is dorsal and lateral.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Present	Regionally restricted	3D	Expression is in the anterior part of subventricular zone.
TS27: main olfactory bulb	Present	Regionally restricted	3D

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present		3E
TS28: main olfactory bulb	Present		3E

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		3F
TS28: brain subventricular zone	Present	Regionally restricted	3F	Expression is in the dorso-lateral wall.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		3G
TS28: main olfactory bulb granule cell layer	Present		3G
TS28: brain subventricular zone	Present	Regionally restricted	3G	Expression is in the anterior part of subventricular zone.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Very strong		4A

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Very strong		4B

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		4C
TS27: cerebellum external granule cell layer	Present		4C
TS27: cerebellum molecular layer	Present	Single cells	4C	Expressing cells migrate through the molecular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		4D
TS27: cerebellum external granule cell layer	Present		4D
TS27: cerebellum molecular layer	Present	Single cells	4D	Expressing cells migrate through the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4E
TS28: cerebellum external granule cell layer	Present		4E
TS28: cerebellum internal granule cell layer	Present		4E
TS28: cerebellum molecular layer	Present	Single cells	4E	Expressing cells migrate through the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4F
TS28: cerebellum external granule cell layer	Present		4F
TS28: cerebellum internal granule cell layer	Present		4F
TS28: cerebellum molecular layer	Present	Single cells	4F	Expressing cells migrate through the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Very strong		4G
TS28: cerebellum Purkinje cell layer	Present		4G

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Very strong		4H
TS28: cerebellum Purkinje cell layer	Present		4H

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Very strong		4I
TS28: cerebellum Purkinje cell layer	Present		4I

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Very strong		4J
TS28: cerebellum Purkinje cell layer	Present		4J

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		5B , 5C , 5A
TS28: main olfactory bulb granule cell layer	Present		5B , 5C , 5A
TS28: rostral migratory stream	Weak	Single cells	5C , 5B , 5A	Staining in the horizontal limb of the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Very strong		5E , 5F , 5D
TS28: hippocampus CA3	Very strong		5E , 5F , 5D
TS28: dentate gyrus granule cell layer	Very strong	Regionally restricted	5F , 5D , 5E	Expression is in the outer shell.