Gene Expression Data
Assay Details
Assay
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Reference:
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J:9556
Lescisin KR, et al., Isolation and characterization of a novel trophoblast-specific cDNA in the mouse. Genes Dev. 1988 Dec;2(12A):1639-46
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3529243
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Gene symbol:
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Tpbpa
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Gene name:
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trophoblast specific protein alpha
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Probe:
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Clone 4311
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Some of the sections used in these studies were cryosections, others were embedded in ester wax. It was not always clear which specimen preparation method was used for the specimens in the figures.
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Results
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Specimen
E6.5 embryo:
embryonic day 6.5
(more )
Specimen
E6.5 embryo:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS9: ectoplacental cone |
Present |
Regionally restricted |
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Expression is present in a a few, isolated cells.
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Specimen
6A/B:
embryonic day 7.5
(more )
Specimen
6A/B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: ectoplacental cone |
Present |
Regionally restricted |
6A/B
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Expression is present in a subset of cells at the mesometrial end.
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Specimen
Maternal decidua:
postnatal
(more )
Note: Days 7.5-10.5 of gestation.
Specimen
Maternal decidua:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Days 7.5-10.5 of gestation.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
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Expression is present in a few, isolated cells.
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Specimen
6D:
embryonic day 8.5
(more )
Specimen
6D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: extraembryonic component |
Present |
Regionally restricted |
6D
,
6C
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Expression is present in outer ectoplacental cone cells, in a region just below the giant cell layer.
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Specimen
6E:
embryonic day 9.5
(more )
Specimen
6E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: extraembryonic component |
Strong |
Regionally restricted |
6E
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Expression is present in outer ectoplacental cone cells, in a region just below the giant cell layer.
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Specimen
6F:
embryonic day 10.5
(more )
Specimen
6F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: extraembryonic component |
Strong |
Regionally restricted |
6F
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Expression is present in outer ectoplacental cone cells, in a region just below the giant cell layer.
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Specimen
7A:
embryonic day 13.5
(more )
Specimen
7A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Strong |
Regionally restricted |
7A
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There is intense, patchy hybridization in the spongiotrophoblast; there is no hybridization to the giant cells or to the labyrinthine layer.
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TS21: extraembryonic component |
Present |
Regionally restricted |
7A
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There is some hybridization of cells in the mesometrial decidual tissue. Other studies suggest these cells are derived from the trophoblast of the conceptus.
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Specimen
E13.5 embryo:
embryonic day 13.5
(more )
Specimen
E13.5 embryo:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 13.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Specimen
7B:
embryonic day 15.5
(more )
Specimen
7B:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 15.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Strong |
Regionally restricted |
7B
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There is intense, patchy hybridization in the spongiotrophoblast; there is no hybridization to the giant cells or to the labyrinthine layer.
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TS23: extraembryonic component |
Present |
Regionally restricted |
7B
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There is some hybridization of cells in the mesometrial decidual tissue. Other studies suggest these cells are derived from the trophoblast of the conceptus.
|
Specimen
E15.5 embryo:
embryonic day 15.5
(more )
Specimen
E15.5 embryo:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 15.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
7C:
embryonic day 18.5
(more )
Specimen
7C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: placenta |
Strong |
Regionally restricted |
7C
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There is intense, patchy hybridization in the spongiotrophoblast; there is no hybridization to the giant cells or to the labyrinthine layer.
|
TS26: extraembryonic component |
Present |
Regionally restricted |
7C
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There is some hybridization of cells in the mesometrial decidual tissue. Other studies suggest these cells are derived from the trophoblast of the conceptus.
|
Specimen
E18.5 embryo:
embryonic day 18.5
(more )
Specimen
E18.5 embryo:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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