Gene Expression Data
Assay Details
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Assay
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Reference:
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J:94401
Brown N, et al., Embryo-uterine interactions via the neuregulin family of growth factors during implantation in the mouse. Biol Reprod. 2004 Dec;71(6):2003-11
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3529135
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Gene symbol:
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Nrg1
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Gene name:
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neuregulin 1
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Probe:
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SMDF cDNA
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2A:
postnatal adult
(more  )
Note: This animal was sacrificed on day 1 of pregnancy.
Specimen
2A:
(close  )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This animal was sacrificed on day 1 of pregnancy.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: endometrium stroma |
Weak |
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2A
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Expression is present in the uterine stroma. No signals were detected in the luminal or glandular epithelium.
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| TS28: endometrium glandular epithelium |
Absent |
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2A
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Expression is present in the uterine stroma. No signals were detected in the luminal or glandular epithelium.
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| TS28: endometrium luminal epithelium |
Absent |
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2A
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Expression is present in the uterine stroma. No signals were detected in the luminal or glandular epithelium.
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Specimen
2B:
postnatal adult
(more )
Note: This animal was sacrificed at 9 am on day 4 of pregnancy.
Specimen
2B:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This animal was sacrificed at 9 am on day 4 of pregnancy.
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Specimen
2C:
postnatal adult
(more )
Note: This animal was sacrificed at midnight on day 4 of pregnancy.
Specimen
2C:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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|
Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This animal was sacrificed at midnight on day 4 of pregnancy.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: endometrium stroma |
Present |
Regionally restricted |
2C
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Expression is present in the uterine stroma immediately beneath the luminal epithelium and surrounding the implanting blastocyst at the antimesometrial pole. Expression does not extend into inter-implantation regions.
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Specimen
2D:
postnatal adult
(more )
Note: This animal was sacrificed on the morning of day 5 of pregnancy.
Specimen
2D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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|
Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
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This animal was sacrificed on the morning of day 5 of pregnancy.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: endometrium stroma |
Present |
Regionally restricted |
2D
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Expression is present in the uterine stroma immediately beneath the luminal epithelium and surrounding the implanting blastocyst at the antimesometrial pole. Expression does not extend into inter-implantation regions.
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Analysis Tools