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Gene Expression Data
Assay Details
Assay
Reference: J:95790 Richard C, et al., Patency of the preterm fetal ductus arteriosus is regulated by endothelial nitric oxide synthase and is independent of vasa vasorum in the mouse. Am J Physiol Regul Integr Comp Physiol. 2004 Sep;287(3):R652-60
Assay type: RT-PCR
MGI Accession ID: MGI:3528083
Gene symbol: Nos1
Gene name: nitric oxide synthase 1, neuronal
Probe: st97_678
Probe preparation: Antisense labelled with P32
Assay notes: A minimum of 30 samples from six litters were represented for each gestational day. RT-PCR products were electrophoresed, transferred to membranes, and subjected to Southern hybridization with gene specific probes. The results were also verified using quantitative PCR approaches.
Results Image: 1A nNOS
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
17 + E16.5 (a) TS24: ductus arteriosus Present Not Specified; total RNA CD-1 Not Specified
17 - Control
18 + E17.5 (a) TS25: ductus arteriosus Present Not Specified; total RNA CD-1 Not Specified
18 - Control
19 + E18.5 (a) TS26: ductus arteriosus Present Not Specified; total RNA CD-1 Not Specified
19 - Control
PP1 + P1 TS27: ductus arteriosus Present Not Specified; total RNA CD-1 Not Specified
PP1 - Control
kid + P adult TS28: metanephros Present Not Specified; total RNA CD-1 Not Specified
m + P adult TS28: skeletal muscle Present Not Specified; total RNA CD-1 Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression is only visible after long exposure times.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory