Gene Expression Data
Assay Details
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Assay
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Reference:
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J:95433
Vrljicak P, et al., Smad expression during kidney development. Am J Physiol Renal Physiol. 2004 Apr;286(4):F625-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3527857
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Gene symbol:
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Smad1
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Gene name:
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SMAD family member 1
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Probe:
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Smad1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
whole mount:
embryonic day 15.0
(more  )
Specimen
whole mount:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: metanephros |
Present |
Regionally restricted |
3_Smad1 whole-mount
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Specimen
cryosection:
embryonic day 15.0
(more )
Specimen
cryosection:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: renal cortex |
Present |
Regionally restricted |
3_Smad1 cryosection
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Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
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| TS23: renal medullary interstitium |
Present |
Regionally restricted |
3_Smad1 cryosection
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Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
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| TS23: ureter |
Present |
Regionally restricted |
3_Smad1 cryosection
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Strongly expressed in the cortex, in uninduced mesenchymal cells in the nephrogenic zone and in ureteric bud tips. A weaker signal was detected in mesenchymal cells in the medulla and around the ureter.
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Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metanephros |
Present |
Regionally restricted |
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Authors report similar results to that obtained at E15.
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Analysis Tools