Gene Expression Data
Assay Details
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Assay
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Reference:
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J:95433
Vrljicak P, et al., Smad expression during kidney development. Am J Physiol Renal Physiol. 2004 Apr;286(4):F625-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3527851
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Gene symbol:
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Smad4
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Gene name:
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SMAD family member 4
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Probe:
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Smad4 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
whole mount:
embryonic day 15.0
(more  )
Specimen
whole mount:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: renal cortex |
Strong |
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2_Smad4 whole-mount
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| TS23: metanephros |
Present |
Regionally restricted |
2_Smad4 whole-mount
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Strongly expressed in the nephrogenic zone. Markedly reduced in the medulla.
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Specimen
cryosection:
embryonic day 15.0
(more )
Specimen
cryosection:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesenchyme of metanephros |
Present |
Regionally restricted |
2_Smad4 cryosection
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Strong signal in the uninduced mesenchyme and the ureteric bud tips within the nephrogenic zone. More weakly expressed by mesenchymal cells in the interstitium. Weakly expressed in the mesenchymal cells surrounding the ureter.
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| TS23: ureter |
Present |
Regionally restricted |
2_Smad4 cryosection
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Strong signal in the uninduced mesenchyme and the ureteric bud tips within the nephrogenic zone. More weakly expressed by mesenchymal cells in the interstitium. Weakly expressed in the mesenchymal cells surrounding the ureter.
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Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metanephros |
Present |
Regionally restricted |
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Authors report similar results to that obtained at E15.
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Analysis Tools