Antibody:	anti-human secretin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	1C top center , 1C top left	Expression in the cephalic mesenchyme in the midbrain region.

Structure	Level	Pattern	Image	Note
TS18: cerebellum primordium	Present		1C bottom left

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	1D top left , 1D top center	Expression in the cephalic mesenchyme in the midbrain region.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		1D bottom left

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		1D bottom center right

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	2C top left , 2C top center right , 2C top center left	Expression in the mesencephalic flexure.
TS22: midbrain tegmentum	Present		2C top left , 2C top center left , 2C top center right

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		2C bottom center , 2C bottom left
TS22: choroid plexus	Present		2C bottom center , 2C bottom left

Structure	Level	Pattern	Image	Note
TS24: midbrain	Present	Regionally restricted	2D top center right , 2D top center left , 2D top left	Expression in the mesencephalic flexure.
TS24: midbrain tegmentum	Present		2D top center right , 2D top left , 2D top center left

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present		2D bottom center right , 2D bottom left , 2D bottom center left
TS24: choroid plexus	Present		2D bottom center right , 2D bottom left , 2D bottom center left

Structure	Level	Pattern	Image	Note
TS18: cardiac muscle tissue	Present		3E left , 3E center
TS18: visceral pericardium	Absent		3E left , 3E center

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present		3F left , 3F center
TS20: visceral pericardium	Present		3F left , 3F center

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Present		3G center , 3G left
TS22: visceral pericardium	Present		3G center , 3G left

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Present		3H left , 3H center
TS24: visceral serosal pericardium	Present		3H left , 3H center

Structure	Level	Pattern	Image	Note
TS22: intestine	Present	Regionally restricted	4C center , 4C left	Expression in the circular muscle layer.

Structure	Level	Pattern	Image	Note
TS24: intestine	Present	Regionally restricted	4D left , 4D center	Expression in the circular muscle layer.

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	5C center , 5C left	Expression in the ureteric bud.
TS22: collecting duct	Present		5C center , 5C left
TS22: maturing glomerular tuft	Present		5C center , 5C left
TS22: mesenchyme of metanephros	Present		5C center , 5C left

Structure	Level	Pattern	Image	Note
TS24: collecting duct	Present	Regionally restricted	5D center , 5D left	Expression in the collecting ducts.
TS24: maturing glomerular tuft	Present		5D left , 5D center