Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Flt1tm2Msh
Name:	FMS-like tyrosine kinase 1; targeted mutation 2, Masabumi Shibuya
MGI ID:	MGI:3527129
Synonyms:	Flt1-TM-TK-, VEGFR1(TM-TK)-
Gene:	Flt1  Location: Chr5:147498414-147662821 bp, - strand  Genetic Position: Chr5, 87.01 cM, cytoband G
Alliance:	Flt1tm2Msh page

Germline Transmission:	Earliest citation of germline transmission: J:95387
Parent Cell Line:	CCE/EK.CCE (ES Cell)
Strain of Origin:	129S/SvEv-Gpi1c

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A neomycin resistance gene replaced exon 16, encoding the transmembrane domain. No clear bands were seen for exons downstream of exon 15 in RT-PCR analysis, showing that the protein was truncated at the termination codon in exon 15. Mutant lung tissues demonstrated that the 110-kDa endogenous soluble form was present, as it results from a termination in intron 13. Also present was a new truncated form, approximately 120-kDa, detected with an antiserum raised against the amino acid sequence of exon 15. (J:95387)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Flt1 Mutation:	73 strains or lines available

Original:	J:95387 Hiratsuka S, et al., Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice. Mol Cell Biol. 2005 Jan;25(1):346-54
All:	3 reference(s)