Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cybanmf333
Name:	cytochrome b-245, alpha polypeptide; neuroscience mutagenesis facility 333
MGI ID:	MGI:3526726
Synonyms:	p22(phox)-, p22phox-
Gene:	Cyba  Location: Chr8:123151515-123159669 bp, - strand  Genetic Position: Chr8, 71.04 cM, cytoband E1
Alliance:	Cybanmf333 page

Strain of Origin:

A.B6-Tyr+/J

Allele Type:    Chemically induced (ENU) Mutation:    Single point mutation   Mutation details: This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the Cyba gene of nmf333 mice revealed a T to C point mutation in exon 5, resulting in a tyrosine to histidine substitution at amino acid position 121 of the encoded protein. The mutated tyrosine is located within a predicted transmembrane domain of the protein and is likely to have profound structural consequences. The identified point mutation did not diminish mRNA expression; RT-PCR analysis revealed that the mutant transcript was present at normal levels in the spleens of mutant mice. However, Western blot experiments demonstrated that mature protein was absent from the neutrophil granulocytes of homozygous nmf333 mice while expression was readily detected in the neutrophils of control littermates. (J:132359) Inheritance:    Recessive

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	52021 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cyba Mutation:	17 strains or lines available

Original:	J:82238 JAX Neuroscience Mutagenesis Facility, Heritable mouse mutants from JAX NMF ENU Mutagenesis Program. MGI Direct Data Submission. 2003;
All:	10 reference(s)