Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Nherf1tm1Geo
Name:	NHERF family PDZ scaffold protein 1; targeted mutation 1, Maria-Magdelena Georgescu
MGI ID:	MGI:3526430
Synonyms:	EBP50-
Gene:	Nherf1  Location: Chr11:115054167-115072007 bp, + strand  Genetic Position: Chr11, 80.65 cM, cytoband E2
Alliance:	Nherf1tm1Geo page

Germline Transmission:	Earliest citation of germline transmission: J:95282
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A targeting vector was designed to replace exons 1-4, which contain most of the coding sequence, with a neo gene lacking the polyadenlylation sequence. RT-PCR and sequencing revealed that exons 5 and 6 were transcribed after the stop codon of the neo mRNA, as part of the 3' UTR. Absence of protein from exons 5 and 6 was further confirmed by Western blot analysis of kidneys from mutant animals. (J:95282)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nherf1 Mutation:	17 strains or lines available

Original:	J:95282 Morales FC, et al., Ezrin-radixin-moesin (ERM)-binding phosphoprotein 50 organizes ERM proteins at the apical membrane of polarized epithelia. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17705-10
All:	5 reference(s)