Gene Expression Data
Assay Details
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Assay
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Reference:
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J:92194
Li Y, et al., Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis. Dev Biol. 2004 Jun 1;270(1):214-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3526076
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Gene symbol:
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Ccnd3
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Gene name:
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cyclin D3
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Antibody:
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anti-cyclin D3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
4Ag:
embryonic day 13.5
(more  )
Specimen
4Ag:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung |
Present |
Regionally restricted |
4A g
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Distinct expression at the periphery of the lung. and throughout mesenchyme.
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| TS21: lung epithelium |
Present |
Regionally restricted |
4A g
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Higher levels in proximal epithelium; lower levels distally.
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| TS21: lung mesenchyme |
Present |
Regionally restricted |
4A g
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Expression throughout mesenchyme. High mesothelium expression. Expression in bronchial smooth muscle region lining the bronchial epithelium.
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Specimen
4Ah:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung |
Present |
Regionally restricted |
4A h
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Authors report that expression was comparable to wild-type, except for a lack of bronchial smooth muscle cells.
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Specimen
4Ai:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3H/HeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung |
Present |
Regionally restricted |
4A i
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Authors report that expression was comparable to wild-type, except for a lack of bronchial smooth muscle cells.
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Analysis Tools
