Probe:	6OST2 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: foregut	Present	Regionally restricted	1B	Expression is detected in the foregut mesoderm but absent in the foregut endoderm.
TS15: septum transversum	Present		1B
TS15: trunk somite	Present		1B

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Absent		1I
TS15: infundibular recess of 3rd ventricle	Absent		1I

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		1K
TS17: infundibular recess of 3rd ventricle	Absent		1K

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		1M

Structure	Level	Pattern	Image	Note
TS19: neural tube basal columns	Weak	Regionally restricted	1P	Low expression is detected in the motor neurons.
TS19: neural tube ventricular layer	Weak		1P
TS19: dorsal root ganglion	Absent		1P

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Absent		2E
TS22: pons	Present	Non-Uniform	2E	Expression is predominantly vebtral.
TS22: choroid plexus	Present		2E
TS22: thalamus	Present		2E

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Absent		2F

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Non-Uniform	2G	Expression is predominantly ventral.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present		2H

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		2I

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	3A	Expression detected proximal to but not in the optic stalk and in the neurepithelium of the diencephalon.
TS15: optic stalk	Present	Regionally restricted	3A	Expression detected proximal to but not in the optic stalk and in the neurepithelium of the diencephalon.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present		3B
TS17: optic stalk	Present	Regionally restricted	3B	Expression detected in the anterior optic stalk.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Strong		3C
TS19: pigmented retinal epithelium	Strong		3C
TS19: surface ectoderm	Strong		3C
TS19: lens vesicle	Strong	Regionally restricted	3C	Expression is detected in the elongated cells along the dorsal aspect of the lens vesicle.

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Strong		3E
TS22: neural retinal epithelium	Absent		3E

Structure	Level	Pattern	Image	Note
TS22: optic nerve	Absent		3H
TS22: extrinsic ocular muscle	Present		3H
TS22: perioptic mesenchyme	Present		3H

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Absent		3L

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present		4B
TS15: neural tube	Absent		4B

Structure	Level	Pattern	Image	Note
TS17: tail myocoele	Present	Regionally restricted	4D	Expression is detected in the myotome adjacent to the differentiating somites.

Structure	Level	Pattern	Image	Note
TS22: pectoral girdle and thoracic body wall musculature	Present	Regionally restricted	4E	Expression detected in the intercostal skeletal muscles.

Structure	Level	Pattern	Image	Note
TS22: tail paraxial mesenchyme	Present	Regionally restricted	4F	Expression detected in the tail muscles.

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Present	Regionally restricted	4G	Expression is detected in the atrial mesoderm adjacent to the septum transversum.

Structure	Level	Pattern	Image	Note
TS15: pericardial component	Present		4H	Expression detected in the pericardium.
TS15: pericardio-peritoneal canal	Present		4H	Expression detected in the pericardium.

Structure	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	Present	Regionally restricted	4I	Expression localized to the myocardial layer adjacent to the intraventricular septum.

Structure	Level	Pattern	Image	Note
TS18: cardiac muscle tissue	Present	Regionally restricted	4J	Expression localized to the myocardial layer adjacent to the intraventricular septum.

Structure	Level	Pattern	Image	Note
TS22: heart atrium	Present		4K
TS22: heart ventricle	Absent		4K

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum mesenchyme	Trace		5B inset , 5B section
TS13: alimentary system	Present	Regionally restricted	5B inset , 5B section	Expression detected in the mesoderm associated with the septum transversum, the mesoderm rectangle of the extraembryonic tissues and the liver primordium.
TS13: extraembryonic component	Present	Regionally restricted	5B inset , 5B section	Expression detected in the mesoderm associated with the septum transversum, the mesoderm rectangle of the extraembryonic tissues and the liver primordium.
TS13: septum transversum	Present	Regionally restricted	5B inset , 5B section	Expression detected in the mesoderm associated with the septum transversum, the mesoderm rectangle of the extraembryonic tissues and the liver primordium.

Structure	Level	Pattern	Image	Note
TS15: hepatic primordium	Strong	Regionally restricted	5D	Strong expression is detected in the epithelium of the liver bud.
TS15: pancreas primordium	Present	Regionally restricted	5D	Expression is detected in the epithelium of the pancreatic primordia.

Structure	Level	Pattern	Image	Note
TS17: hepatic primordium parenchyma	Absent		5F
TS17: trunk mesenchyme	Present	Regionally restricted	5F	Expression present in the muscles of the diaphragm.
TS17: liver	Present	Regionally restricted	5F	Expression detected in the mesothelial cells of the liver capsule.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Weak	Regionally restricted	5H	Expression is detected in the pancreas mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: branchial arch endoderm	Present	Regionally restricted	5J	Expression does not extend beyond the 3rd Pharyngeal pouch.
TS15: branchial arch mesenchyme	Weak	Regionally restricted	5J	Expression does not extend beyond the 3rd Pharyngeal pouch.

Structure	Level	Pattern	Image	Note
TS15: thyroid primordium	Absent		5L

Structure	Level	Pattern	Image	Note
TS17: esophagus epithelium	Absent		6C
TS17: trachea epithelium	Absent		6C
TS17: embryo mesenchyme	Present	Regionally restricted	6C	Present in the mesenchyme surrounding the oesophagus and trachea with higher levels ventrally.

Structure	Level	Pattern	Image	Note
TS17: lung mesenchyme	Present		6F

Structure	Level	Pattern	Image	Note
TS18: esophagus epithelium	Strong		6G

Structure	Level	Pattern	Image	Note
TS22: tooth epithelium	Present		6K
TS22: tooth mesenchyme	Absent		6K

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	6N	Expression detected in the mesenchyme of the vibrissae.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Uniform	7B
TS15: hindlimb ridge mesenchyme	Present	Uniform	7B

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	7E section	Expression is detected in the proximal forelimb mesenchyme in an anterior and posterior domain

Structure	Level	Pattern	Image	Note
TS22: limb digit	Strong	Non-Uniform	7M section , 7N	Strong expression is detected in the skeletal muscle of the developing digits with lower expression in the joints and perichondrium.

Structure	Level	Pattern	Image	Note
TS16: mesonephros mesenchyme	Present		8B
TS16: gonad primordium	Weak	Regionally restricted	8B	Expression is detected in the genital ridge.

Structure	Level	Pattern	Image	Note
TS17: mesonephros mesenchyme	Present		8D
TS17: gonad primordium	Weak	Regionally restricted	8D	Weak expression detected in the genital ridge.

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present	Regionally restricted	8F	Expression detected in the peripheral mesenchyme of the kidney.

Structure	Level	Pattern	Image	Note
TS22: gonad	Present		8H section

Structure	Level	Pattern	Image	Note
TS13: head somite	Strong
TS13: trunk somite	Strong