Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:93571
Rowan S, et al., Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10. Development. 2004 Oct;131(20):5139-52
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3525848
|
|
Gene symbol:
|
Wfdc1
|
|
Gene name:
|
WAP four-disulfide core domain 1
|
|
Probe:
|
Wfdc1 EST
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
E14.5 heterozygote:
embryonic day 14.5; Vsx2or-J/Vsx2 +
(more  )
Specimen
E14.5 heterozygote:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6 * FVB/N * SJL
|
|
Mutant Allele(s):
|
Vsx2or-J/Vsx2+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
Specimen
E14.5 homozygote:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6 * FVB/N * SJL
|
|
Mutant Allele(s):
|
Vsx2or-J/Vsx2or-J
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
3E E14.5 orJ/orJ
|
Expression is expanded centrally compared to what is seen in wild type animals.
|
Specimen
E17.5 heterozygote:
embryonic day 17.5; Vsx2or-J/Vsx2 +
(more )
Specimen
E17.5 heterozygote:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * FVB/N * SJL
|
|
Mutant Allele(s):
|
Vsx2or-J/Vsx2+
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
Specimen
E17.5 homozygote:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6 * FVB/N * SJL
|
|
Mutant Allele(s):
|
Vsx2or-J/Vsx2or-J
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: retina |
Present |
Regionally restricted |
3E E17.5 orJ/orJ
|
Thre is expression in some cells in the center of the retina, as well as in peripheral parts of the retina that have not yet undergone transdifferentiation.
|
|
Analysis Tools