Sry RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:23597 Cao QP, et al., Expression of the mouse testis-determining gene Sry in male preimplantation embryos. Mol Reprod Dev. 1995 Feb;40(2):196-204
Assay type:	RT-PCR
MGI Accession ID:	MGI:3524910
Gene symbol:	Sry
Gene name:	sex determining region of Chr Y

Probe:	Pr3, Pr5
Probe preparation:	Antisense labelled with P32 RNA
Assay notes:	This is an RT/PCR/RNase assay: total RNA was reverse transcribed and amplified by PCR. The amplified fragments were detected by their ability to protect a riboprobe from RNase digestion. The probe used is pSry2 (MGI:25407).

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
P	Control
+ lb	E4.0 (a)	TS5: blastocyst-stage conceptus	Present	Not Specified; total RNA	involves: C57BL/6 * CF-1 * SJL		Not Specified	Three embryo equivalents.
- lb	Control
+ mb	E4.0 (b)	TS5: blastocyst-stage conceptus	Present	Not Specified; total RNA	involves: C57BL/6 * CF-1 * SJL		Not Specified	Three embryo equivalents.
- mb	Control
+ eb	E4.0 (c)	TS5: blastocyst-stage conceptus	Present	Not Specified; total RNA	involves: C57BL/6 * CF-1 * SJL		Not Specified	Three embryo equivalents.
- eb	Control
d	Control
M	Control

Notes:
(a) Late blastocyst stage. Age of embryo at noon of plug day not specified in reference.
(b) Mid blastocyst stage. Age of embryo at noon of plug day not specified in reference.
(c) Early blastocyst stage. Age of embryo at noon of plug day not specified in reference.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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