Gene Expression Data
Assay Details
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Assay
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Reference:
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J:94397
Chan R, et al., Modulation of Erbb2 signaling during development: a threshold level of Erbb2 signaling is required for development. Development. 2004 Nov;131(22):5551-60
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3522599
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Gene symbol:
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Phox2a
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Gene name:
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paired-like homeobox 2a
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Probe:
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Phox2a probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Fixation was in 4% paraformaldehyde/0.2% gluteraldehyde.
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Results
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Specimen
erbB2:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Erbb2tm4(Erbb2)Mul/Erbb2+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
erbB2:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Erbb2tm4(Erbb2)Mul/Erbb2tm1Haus
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: sympathetic ganglion |
Present |
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3B
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In this mutant, there is only partial development of the thoracic ganglia and the defects become more severe in a rostral-caudal gradient.
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Specimen
erbB2:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Erbb2tm5(Erbb2)Mul/Erbb2+
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
erbB2:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Erbb2tm5(Erbb2)Mul/Erbb2tm1Haus
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: sympathetic ganglion |
Present |
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3D
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Expression in this mutant is similar to that of the heterozygous littermates.
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Analysis Tools
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