Antibody:	anti-Cdx2
Detection system:	Secondary antibody
Assay notes:	The tissues used in this assay were fixed in methacarn.

Structure	Level	Pattern	Image	Note
TS28: germ cell of ovary	Absent			There is no expression in the germ cells of the ovary at any stage of maturation.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Absent			There was some brown staining in the blastomere cytoplasm, but this was thought to be non-specific since it is present in sections processed without the primary antibody.

Structure	Level	Pattern	Image	Note
TS3: 4-8 cell stage embryo	Absent			There was some brown staining in the blastomere cytoplasm, but this was thought to be non-specific since it is present in sections processed without the primary antibody.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present		1	Expression is present in the nuclei.
TS4: inner cell mass	Absent		1

Structure	Level	Pattern	Image	Note
TS5: trophectoderm	Present		2	Expression is present in the nuclei.
TS5: inner cell mass	Absent		2

Structure	Level	Pattern	Image	Note
TS7: polar trophectoderm	Present	Regionally restricted	3	Expression is present in the nuclei of the extra-embryonic ectoderm adjacent to the embryoblast.
TS7: embryo	Absent		3
TS7: extraembryonic endoderm	Absent		3
TS7: mural trophectoderm	Absent		3

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Strong	Regionally restricted	4	The extra-embryonic ectoderm lying next to the embryoblast was strongly positive.
TS9: definitive endoderm	Absent		4
TS9: ectoplacental cone	Absent		4
TS9: embryo	Absent		4
TS9: embryo ectoderm	Absent		4
TS9: extraembryonic endoderm	Absent		4
TS9: extraembryonic visceral endoderm	Absent		4
TS9: parietal endoderm	Absent		4
TS9: polar trophectoderm	Absent		4
TS9: primary trophoblast giant cell	Absent		4

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Present		5	Expression is present in the nuclei.
TS11: ectoplacental cone	Present		5	Expression is present in the nuclei surrounding the ectoplacental canal, as well as in trophectodermal cels on the mesometrial aspect of the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS11: allantois mesoderm	Present		6
TS11: chorion ectoderm	Present		6
TS11: amnion ectoderm	Present	Regionally restricted	6	Expression is present in a few cells adjacent to the allantoic bud.
TS11: embryo	Absent		6

Structure	Level	Pattern	Image	Note
TS11: cytotrophoblast	Present		7	Expression is present in the ectoplacental glycogen cells; the remainder of the ectoplacental cone is negative.
TS11: primary trophoblast giant cell	Absent		7

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Uniform	8	Expression is in all tissues of the tail bud.
TS14: extraembryonic component	Present	Regionally restricted	8	Expression is present in the root of the allantois.
TS14: hindgut diverticulum epithelium	Present		8

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Present
TS14: neural tube	Present
TS14: notochord	Present	Regionally restricted		Expression is present in the notochord caudal to somite 6.
TS14: head somite	Absent
TS14: intermediate mesenchyme	Absent
TS14: mesenchyme derived from lateral plate	Absent
TS14: trunk somite	Absent

Structure	Level	Pattern	Image	Note
TS14: extraembryonic component	Present	Regionally restricted	9	Expression is present in the lower layer of the lamina and the ectoplacental glycogen cells situated in the layer adjacent to the trophoblastic giant cells. All other extra-embryonic structures are negative.
TS28: placenta	Present	Regionally restricted	9	Expression is present in the lower layer of the lamina and the ectoplacental glycogen cells situated in the layer adjacent to the trophoblastic giant cells. All other extra-embryonic structures are negative.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Graded	10	Expression is present in the nuclei. This staining gradually disappears at a more caudal level.
TS15: notochord	Strong	Graded	10	Expression is present in the nuclei. This staining gradually disappears at a more caudal level.
TS15: hindgut	Strong		10	Expression is present in the nuclei.

Structure	Level	Pattern	Image	Note
TS15: gut	Present	Regionally restricted	11	Expression begins at a sharp demarcation line just cranial to the junction of the foregut and midgut.

Structure	Level	Pattern	Image	Note
TS15: gut	Present		12
TS15: neural tube	Present		12
TS15: tail unsegmented mesenchyme	Present		12	Expression is present in the mesoderm of the tail bud.
TS15: trunk somite	Absent		12

Structure	Level	Pattern	Image	Note
TS15: extraembryonic component	Strong	Regionally restricted	13	Expression is present in the nuclei of the spongy cytotrophoblast cells, while the more superficial trophoblastic giant cells and the deeper cells of the labyrinth are negative.
TS28: placenta	Strong	Regionally restricted	13	Expression is present in the nuclei of the spongy cytotrophoblast cells, while the more superficial trophoblastic giant cells and the deeper cells of the labyrinth are negative.

Structure	Level	Pattern	Image	Note
TS20: gut	Strong	Regionally restricted	14	Expression is present in the epithelial lining of the gut proximal and distal to the hepatic diverticulum.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	15	Expression is present in the epithelial lining of the gut, although not in regions occurring cranial to the hepatic diverticulum.

Structure	Level	Pattern	Image	Note
TS20: midgut	Present		16
TS20: midgut derived duodenum	Present		16

Structure	Level	Pattern	Image	Note
TS20: hindgut	Present	Graded	17	Expression is present in the hindgut, although staining is diminished in the distal hindgut when compared to more proximal regions; there is no staining in the rectum.
TS20: urogenital sinus	Absent		17	There is no staining in the epithelium.

Structure	Level	Pattern	Image	Note
TS20: extraembryonic component	Present	Regionally restricted	18	Expression is present in the spongy cytotrophoblast cells lying superficial to the placental labyrinth and in the yolk sac stalk.
TS28: placenta	Present	Regionally restricted	18	Expression is present in the spongy cytotrophoblast cells lying superficial to the placental labyrinth and in the yolk sac stalk.

Structure	Level	Pattern	Image	Note
TS20: tail central nervous system	Present	Regionally restricted		There is expression in the nuclei in the neural tube at the very tip of the tail.