Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67054
Laurikkala J, et al., TNF signaling via the ligand-receptor pair ectodysplasin and edar controls the function of epithelial signaling centers and is regulated by Wnt and activin during tooth organogenesis. Dev Biol. 2001 Jan 15;229(2):443-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3513991
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Gene symbol:
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Edar
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Gene name:
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ectodysplasin-A receptor
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Probe:
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dl probe2
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Probe preparation:
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labelled with S35 RNA
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Results
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Specimen
1B:
embryonic day 10.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1D:
embryonic day 11.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: oral region |
Strong |
Regionally restricted |
1D
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During initiation of tooth development, intense expression in thickened presumptive dental epithelium.
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Specimen
1F:
embryonic day 12.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: oral region |
Present |
Regionally restricted |
1F
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During early bud stage, expression restricted to budding cells; absent in dental epithelial cells contacting oral epithelium.
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Specimen
1H:
embryonic day 13.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: enamel knot |
Strong |
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1H
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At bud stage, intense expression restricted to tip of tooth bud, corresponding to forming enamel knot.
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Specimen
1J:
embryonic day 14.5
(more )
Specimen
1J:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: enamel organ |
Strong |
Regionally restricted |
1J
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At cap stage, intense expression in enamel knot; absent in all other dental cells.
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Specimen
1L:
embryonic day 17.5
(more )
Specimen
1L:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: enamel organ |
Present |
Regionally restricted |
1L
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Some expression in enamel epithelium in intercuspal area.
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Specimen
1N:
postnatal newborn
(more )
Specimen
1N:
(close )
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Genetic Background:
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NMRI
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: tooth |
Absent |
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1N
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No expression detected in tooth germs.
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: oral region |
Present |
Regionally restricted |
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Authors report expression was unaltered in dental epithelium, compared with wild type.
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Specimen
5G:
embryonic day 13.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tooth epithelium |
Strong |
Regionally restricted |
5G
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Intense and restricted signal at the tip of tooth buds.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: tooth epithelium |
Strong |
Regionally restricted |
5H
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Expression was unaltered in dental epithelium, compared with wild type. Intense and restricted signal at the tip of tooth buds
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Specimen
6G:
embryonic day 14.5
(more )
Specimen
6G:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: enamel knot |
Strong |
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6G
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Intense expression in enamel knot.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: enamel knot |
Strong |
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6H
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Expression in enamel knot is unaffected compared with wild type.
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