Igf1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:86408 Villalpando I, et al., Insulin-like growth factor I (IGF-I) regulates endocrine activity of the embryonic testis in the mouse. J Steroid Biochem Mol Biol. 2003 Aug;86(2):151-8
Assay type:	Western blot
MGI Accession ID:	MGI:3513086
Gene symbol:	Igf1
Gene name:	insulin-like growth factor 1

Antibody:	anti-somatomedin-C
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	31 kDa (b)	Genetic Background	Mutant Allele(s)	Sex
Lane 1	E12.5 (a)	TS20: testis	Present	CD-1		Male
13	E13.5 (a)	TS21: testis	Present	CD-1		Male
14	E14.5 (a)	TS22: testis	Present	CD-1		Male
15	E15.5 (a)	TS23: testis	Present	CD-1		Male
16	E16.5 (a)	TS24: testis	Present	CD-1		Male
17	E17.5 (a)	TS25: testis	Present	CD-1		Male

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) The authors stated that the antibody recognizes five bands with molecular weights ranging from 100 to 31 kDa, but only the 31kDa band was discussed. Its expression level was lowest at E12.5-13.5, but then increased at E14.5 and remained constant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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