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Gene Expression Data
Assay Details
Assay
Reference: J:88272 Dintilhac A, et al., A conserved non-homeodomain Hoxa9 isoform interacting with CBP is co-expressed with the 'typical' Hoxa9 protein during embryogenesis. Gene Expr Patterns. 2004 Mar;4(2):215-22
Assay type: RT-PCR
MGI Accession ID: MGI:3513041
Gene symbol: Hoxa9
Gene name: homeobox A9
Probe: Hoxa9-pG, Hoxa9-pH
Visualized with: Ethidium bromide
Assay notes: The PCR products were Southern blotted with a probe corresponding to nucleotides 1760-1951 located in exon 2. The quantification of the hybridization signals are shown. The results of RT-PCR are detailed in Figure 3A (MGI:3512669).
Results
Image: 3B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
-RT Control
Genital tract E12.5 (b) TS20: reproductive system Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Liver E12.5 (b) TS20: liver Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Forelimb E12.5 (b) TS20: forelimb Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Tail E12.5 (b) TS20: tail Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Genital tract E17.5 (b) TS25: reproductive system Present Strong (c) 0.5 µg; total RNA Not Specified Not Specified
Liver E17.5 (b) TS25: liver Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Forelimb E17.5 (b) TS25: forelimb Present Strong (c) 0.5 µg; total RNA Not Specified Not Specified
Tail E17.5 (b) TS25: tail Present Strong (c) 0.5 µg; total RNA Not Specified Not Specified
Kidney E17.5 (b) TS25: metanephros Present Strong (c) 0.5 µg; total RNA Not Specified Not Specified
Genital tract P8 TS28: reproductive system Not Specified Not Specified 0.5 µg; total RNA Not Specified Female
Liver P adult TS28: liver Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Bone Marrow P adult TS28: bone marrow Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Thymus P adult TS28: thymus Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Spleen P adult TS28: spleen Not Specified Not Specified 0.5 µg; total RNA Not Specified Not Specified
Notes:
(a) This smaller product represents an alternative transcript.
(b) Age of embryo at noon of plug day not specified in reference.
(c) Quantification showed that the alternative transcript is two-fold more abundant.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory